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• W144328554 abstract "Cloning of complementary DNAs (cDNAs), which are predicted to encode G protein-coupled receptors (GPCRs), required a mechanism to ascertain if the single polypeptide encoded by such cDNAs was sufficient to generate the pharmacology and function anticipated for the receptor in question. Because this generally was the case—and despite evidence of greater complexity (reviewed in ref. 1)—it became axiomatic that GPCRs were single, seven-transmembrane-span polypeptides. However, a series of immunoblotting studies has suggested that a fraction of cellular GPCRs might exist as dimers or higher order species in both transfected cell lines and native tissues (2–4). The known propensity of hydrophobic proteins to aggregate—particularly when samples were heated prior to separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)—meant that it was possible to disregard these data. However, studies in which differentially epitope-tagged forms of a single GPCR could be co-immunoprecipitated following their co-expression in heterologous cell lines provided considerable evidence for the presence of dimeric or, indeed, higher oligomeric complexes (2,5). This was generally not observed when the two forms of the GPCR were expressed in separate cell populations that were mixed prior to membrane solubilization and immunoprecipitation. These results indicate that co-immunoprecipitation did not result simply from aggregation of the hydrophobic transmembrane elements of these polypeptides following removal of lipid by treatment with detergents. Equivalent studies then began to examine the proclivity of different, co-expressed GPCRs to be co-immunoprecipitated. These studies have produced a large body of evidence supporting the capacity of GPCRs to exist as heterodimers/-oligomers (for review, see refs. 6 and 7). However, studies that have reported a very broad capacity of particular GPCRs to allow co-immunoprecipitation of co-expressed GPCRs (8) have questioned the relevance of GPCR co-immunoprecipitation data when this is not accompanied by similar data produced by alternative strategies." @default.
• W144328554 created "2016-06-24" @default.
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• W144328554 date "2005-01-01" @default.
• W144328554 modified "2023-10-01" @default.
• W144328554 title "Functional Complementation and the Analysis of GPCR Dimerization" @default.
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• W144328554 doi "https://doi.org/10.1007/978-1-59259-919-6_12" @default.
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