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- W144380056 abstract "pAd(vantage) is a rapid cloning system for generating recombinant adenoviruses. The system is based on manipulating the full-length adenovirus genome as a stable plasmid in E. coli using intron-encoded endonucleases. These intron-encoded endonucleases cut their recognition sequences, which range from 15-39 bp, with high specificity. Their unusual long homing sequence makes them rare-cutting and ideal for use as cloning sites. We report how transgenes can easily be cloned directly into the E1 region of an adenoviral plasmid, followed by transfection into a mammalian packaging cell line, to produce homogeneous recombinant viruses without the need for plaque purification." @default.
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- W144380056 date "1999-03-01" @default.
- W144380056 modified "2023-09-25" @default.
- W144380056 title "Novel Cloning Method for Recombinant Adenovirus Construction in Escherichia coli" @default.
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- W144380056 doi "https://doi.org/10.2144/99263rr01" @default.
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