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- W1444128949 abstract "This chapter focuses on carotene synthesis in capsicum chromoplasts. The carotene biosynthesis involves primarily the condensation of an allylic pyrophosphate to the olefinic isopentenyl pyrophosphate mediated by a prenyltransferase. Carotene composition also described in the chapter. The method described under “Carotene Composition” gives radiochemically pure carotenes for liquid scintillation counting. The unmetabolized and labeled isopentenyl pyrophosphate remains in the water phase during the extraction procedure. Prenols or prenyl pyrophosphate formed during the incubation remains at the origin of the silica gel G chromatoplate developed with light petroleum : diethyl ether. Chromoplasts isolated from this system efficiently incorporate terpene precursors into carotenes while xanthophylls are poorly labeled. This chapter summarizes some of the properties of cell-free system. Intact chromoplasts are prepared from orange pericarp tissue (2 kg) of Capsicum annuum L. fruits. The procedure is described in figure given in the chapter. The purity of the chromoplasts prepared as described in the chapter is verified by several points." @default.
- W1444128949 created "2016-06-24" @default.
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- W1444128949 date "1985-01-01" @default.
- W1444128949 modified "2023-09-25" @default.
- W1444128949 title "[28] Carotene synthesis in Capsicum chromoplasts" @default.
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- W1444128949 doi "https://doi.org/10.1016/s0076-6879(85)10081-9" @default.
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