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• W1444453802 abstract "Publisher Summary This chapter discusses in situ hybridization that has emerged as a powerful tool for the examination of cell type-specific gene expression in the human central nervous system (CNS). The use of human brain tissue for in situ hybridization studies poses special problems that require protection of laboratory personnel from infectious agents, knowledge of patient history, and optimization of technical sensitivity. Despite the presence of endogenous ribonuclease, a consensus of recent experimental data shows that brain mRNA remains stable in intact brain tissue over lengthy postmortem intervals. Morphological sampling should be used to characterize tissue samples destined for biochemical analysis. One of the most trivial problems faced by the novice attempting in situ hybridization methods is maintaining adherence of tissue sections to slides during the procedure. Synthetic oligonucleotide probes offer certain advantages over RNA probes in that they are easy to generate, their small size allows for greater penetration into the tissue, and they do not show some of the problems associated with certain RNA probes. A measurement of relative mRNA abundance between different neuronal subpopulations can be made both by densitometric measurement and by manual grain counting." @default.
• W1444453802 created "2016-06-24" @default.
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• W1444453802 date "1989-01-01" @default.
• W1444453802 modified "2023-09-27" @default.
• W1444453802 title "In Situ Hybridization Approaches to Human Neurological Disease" @default.
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• W1444453802 doi "https://doi.org/10.1016/b978-0-12-185251-1.50015-5" @default.
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