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- W144452009 abstract "Bowman Birk Inhibitor (BBI) is a trypsin/chymotrypsin inhibitor originally prepared from soybean extract that has been implicated as a potential treatment for certain forms of cancer and neuromuscular disease. At present, BBI is not commercially available in a form suitable for this purpose. In this paper, we describe a scalable method that has been used to prepare highly purified BBI in gram quantities. A BBI enriched soy processing stream was used as starting material for purification. The stream was adjusted to pH 3.0, clarified by centrifugation at 4000×g for 10 min, and then applied to a 21.6 × 5 cm column of SP Sepharose Fast Flow resin (GE Healthcare) at 50 ml/min. After washing unbound material away, BBI was eluted with 75 mM Na Citrate, pH 3.0 buffer, concentrated using tangential flow filtration (10 kDa MWCO membrane), then diafiltered to remove buffer salts. Final purification was achieved using Triton X114 detergent to remove endotoxin. This procedure produced approximately 3.6 gms of >;95% pure BBI (as estimated by SDS-PAGE) with a specific activity of 1687 chymotrypsin inhibition units/gram of protein." @default.
- W144452009 created "2016-06-24" @default.
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- W144452009 date "2013-04-01" @default.
- W144452009 modified "2023-09-25" @default.
- W144452009 title "Method for the Preparation of Gram Quantities of Highly Purified Bowman Birk Inhibitor from Glycine max" @default.
- W144452009 doi "https://doi.org/10.1096/fasebj.27.1_supplement.574.2" @default.
- W144452009 hasPublicationYear "2013" @default.
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