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- W1444616081 abstract "This chapter discusses the use of high-density cDNA grids for hybridization fingerprinting experiments. Hybridization fingerprinting experiments with short oligonucleotides as probes provide a method to screen efficiently at relatively low costs without losing much information. It could be shown that the analysis of whole genomes with this technique is viable using automated protocols at various steps. The chapter presents a protocol for the production of high-density grids for the purpose. It explores a number of built-in controls to judge their quality. And a number of possible applications are also listed here. The protocol outlines construction of cDNA libraries, PCR amplification, and production of high-density cDNA grids. The discussion closes with a conclusion that despite some shortcomings of the technique, hybridization fingerprinting is a powerful method to very efficiently extract valuable information about large quantities of cDNA material. Attaining a survey of the mRNA expression pattern of a cell in as little as 6 weeks (including production of library, hybridization, and data analysis) is an achievement which is unrivaled by other experimental approaches." @default.
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- W1444616081 date "1999-01-01" @default.
- W1444616081 modified "2023-10-16" @default.
- W1444616081 title "High-density cDNA Grids for hybridization fingerprinting experiments" @default.
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- W1444616081 doi "https://doi.org/10.1016/b978-012372185-3/50030-4" @default.
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