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- W1444627802 abstract "This chapter describes applications of polyacrylamide gel electrofocusing and electrophoresis in cytoskeletal and contractile protein research, and selected examples of different uses of these techniques. Contractile and cytoskeletal proteins are present at high levels within the cell, and often they are not associated with an easily assayable enzymatic activity. By using polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE), one can detect simultaneously actin, α-actinin, myosin heavy and light chains, tropomyosin, and troponin in skeletal and smooth muscle myofibril preparations. SDS–polyacrylamide gel electrophoresis and isoelectric focusing have proved extremely valuable in assaying the presence of families of contractile and cytoskeletal proteins, in determining the complexity of these multi component families, in studying the developmental regulation of the expression of these proteins, and in assessing their degree of post-translational modification." @default.
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- W1444627802 date "1983-01-01" @default.
- W1444627802 modified "2023-10-14" @default.
- W1444627802 title "Chapter 9.11 Contractile and Cytoskeletal Proteins" @default.
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- W1444627802 doi "https://doi.org/10.1016/s0301-4770(08)61317-2" @default.
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