FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W14459570> ?p ?o ?g. }

• W14459570 abstract "Mitosis is the process by which eukaryotic cells segregate their chromosomes before division. A critical stage of mitosis is anaphase, when the microtubule-based spindle segregates chromosomes into mother and daughter cells prior to cytokinesis. My dissertation research aimed to provide a better understanding of the regulation of anaphase progression and spindle function during mitosis in Saccharomyces cerevisiae. My research focused on two microtubule-associated proteins, Slk19 (CENP-F homolog) and Stu1 (CLASP homolog). These proteins play fundamental roles in anaphase progression, Stu1 in microtubule spindle stability and Slk19 in spindle midzone organization and Cdc14 phosphatase regulation. I found that Stu1 and Slk19 physically interact and that Slk19 regulates Stu1 localization during anaphase. In addition to its interaction with Slk19, I identified a number of other physical and genetic interactors of Stu1 through mass spectrometry, yeast two-hybrid and synthetic genetic analyses. These interactors provide insight into the role of Stu1 at kinetochores. I also investigated Stu1 function through a protein truncation analysis and purification of full-length Stu1 protein. My truncation analysis revealed that the Stu1 C-terminus is dispensable for viability but is necessary for proper protein localization. The N-terminus, however, is essential for viability. My attempts to purify Stu1 from insect cells were partially successful; the protein is extremely sensitive to proteolytic degradation, and under conditions that limit proteolysis, the protein appears to aggregate or oligomerize in solution.I also investigated the role of Slk19 sumoylation in anaphase progression. I determined that the Cdc14 early anaphase release (FEAR) network protein Slk19 is sumoylated in vivo and that sumoylation is important for restricting Cdc14 phosphatase localization to the nucleus at the end of anaphase. A slk19 sumoylation mutant causes premature Cdc14 movement from the nucleus to the bud neck, which affects mitotic exit, as this slk19 sumoylation mutant can partially rescue the spindle disassembly defect of the mitotic exit network mutant cdc15-2 . This slk19 mutant also has aberrant spindle elongation dynamics, which might be due to a change in Cdc14 function during anaphase. In conclusion, my dissertation research has uncovered a number of previously unrecognized interactions among mitotic proteins and has revealed a novel function of sumoylation in the regulation of Cdc14 function during anaphase through the FEAR network protein Slk19." @default.
• W14459570 created "2016-06-24" @default.
• W14459570 creator A5058744756 @default.
• W14459570 date "2011-01-01" @default.
• W14459570 modified "2023-09-27" @default.
• W14459570 title "Regulation of mitotic progression in Saccharomyces cerevisiae by the microtubule-associated proteins Slk19 and Stu1" @default.
• W14459570 cites W1221688681 @default.
• W14459570 cites W1482789507 @default.
• W14459570 cites W1483030389 @default.
• W14459570 cites W1525575010 @default.
• W14459570 cites W1546492519 @default.
• W14459570 cites W1592167581 @default.
• W14459570 cites W1765359824 @default.
• W14459570 cites W1798268191 @default.
• W14459570 cites W1903792504 @default.
• W14459570 cites W1964865474 @default.
• W14459570 cites W1966072209 @default.
• W14459570 cites W1966521684 @default.
• W14459570 cites W1967000365 @default.
• W14459570 cites W1967163876 @default.
• W14459570 cites W1967955373 @default.
• W14459570 cites W1971909950 @default.
• W14459570 cites W1980166711 @default.
• W14459570 cites W1983400295 @default.
• W14459570 cites W1983412918 @default.
• W14459570 cites W1985001103 @default.
• W14459570 cites W1986955288 @default.
• W14459570 cites W2004180261 @default.
• W14459570 cites W2004863845 @default.
• W14459570 cites W2006389406 @default.
• W14459570 cites W2008187459 @default.
• W14459570 cites W2010026263 @default.
• W14459570 cites W2011902848 @default.
• W14459570 cites W2011921894 @default.
• W14459570 cites W2012807621 @default.
• W14459570 cites W2013947447 @default.
• W14459570 cites W2014114945 @default.
• W14459570 cites W2017746827 @default.
• W14459570 cites W2019907883 @default.
• W14459570 cites W2023348733 @default.
• W14459570 cites W2027329338 @default.
• W14459570 cites W2028973684 @default.
• W14459570 cites W2029041966 @default.
• W14459570 cites W2029189334 @default.
• W14459570 cites W2030258596 @default.
• W14459570 cites W2031198628 @default.
• W14459570 cites W2034411357 @default.
• W14459570 cites W2039737272 @default.
• W14459570 cites W2048054554 @default.
• W14459570 cites W2048193644 @default.
• W14459570 cites W2049438326 @default.
• W14459570 cites W2053060343 @default.
• W14459570 cites W2060232163 @default.
• W14459570 cites W2061409915 @default.
• W14459570 cites W2068022037 @default.
• W14459570 cites W2072509928 @default.
• W14459570 cites W2073667083 @default.
• W14459570 cites W2073682421 @default.
• W14459570 cites W2075351483 @default.
• W14459570 cites W2076217757 @default.
• W14459570 cites W2079321797 @default.
• W14459570 cites W2081237677 @default.
• W14459570 cites W2082692750 @default.
• W14459570 cites W2084117490 @default.
• W14459570 cites W2085165779 @default.
• W14459570 cites W2085671161 @default.
• W14459570 cites W2086283062 @default.
• W14459570 cites W2088930180 @default.
• W14459570 cites W2088939436 @default.
• W14459570 cites W2091502861 @default.
• W14459570 cites W2091602394 @default.
• W14459570 cites W2091735806 @default.
• W14459570 cites W2091912506 @default.
• W14459570 cites W2092989086 @default.
• W14459570 cites W2094812080 @default.
• W14459570 cites W2099787706 @default.
• W14459570 cites W2102771118 @default.
• W14459570 cites W2103911091 @default.
• W14459570 cites W2105203156 @default.
• W14459570 cites W2105430792 @default.
• W14459570 cites W2105989492 @default.
• W14459570 cites W2108228282 @default.
• W14459570 cites W2110548948 @default.
• W14459570 cites W2110799552 @default.
• W14459570 cites W2116922645 @default.
• W14459570 cites W2118989130 @default.
• W14459570 cites W2119025394 @default.
• W14459570 cites W2120125134 @default.
• W14459570 cites W2120623923 @default.
• W14459570 cites W2120841896 @default.
• W14459570 cites W2123256728 @default.
• W14459570 cites W2123327064 @default.
• W14459570 cites W2123631689 @default.
• W14459570 cites W2126277736 @default.
• W14459570 cites W2126969704 @default.
• W14459570 cites W2128565407 @default.
• W14459570 cites W2128973947 @default.
• W14459570 cites W2129328366 @default.
• W14459570 cites W2133503471 @default.
• W14459570 cites W2137984821 @default.

• next