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• W1446286296 abstract "Research Article1 August 1993free access Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96. P. Grandi P. Grandi EMBL, Heidelberg, Germany. Search for more papers by this author V. Doye V. Doye EMBL, Heidelberg, Germany. Search for more papers by this author E.C. Hurt E.C. Hurt EMBL, Heidelberg, Germany. Search for more papers by this author P. Grandi P. Grandi EMBL, Heidelberg, Germany. Search for more papers by this author V. Doye V. Doye EMBL, Heidelberg, Germany. Search for more papers by this author E.C. Hurt E.C. Hurt EMBL, Heidelberg, Germany. Search for more papers by this author Author Information P. Grandi1, V. Doye1 and E.C. Hurt1 1EMBL, Heidelberg, Germany. The EMBO Journal (1993)12:3061-3071https://doi.org/10.1002/j.1460-2075.1993.tb05975.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The essential C-terminal domain of NSP1 mediates assembly into the nuclear pore complex (NPC). To identify components which interact physically with this yeast nucleoporin, the tagged C-terminal domain of NSP1 (ProtA-NSP1) was isolated by affinity chromatography under non-denaturing conditions. The purified complex contains ProtA-NSP1, two previously identified ‘GLFG’ nucleoporins, NUP49 (NSP49) and p54 and a novel protein designated NIC96 (for Nucleoporin-Interacting Component of 96 kDa). Conversely, affinity purification of tagged NSP49 enriches for NSP1, the p54 and the NIC96 component. The NIC96 gene was cloned; it encodes a novel 839 amino acid protein essential for cell growth. By immunofluorescence, protein A-tagged NIC96 exhibits a punctate nuclear membrane staining indicative of nuclear pore location. Therefore, affinity purification of tagged nucleoporins has allowed the definition of a subcomplex of the NPC and analysis of physical interactions between nuclear pore proteins. Previous ArticleNext Article Volume 12Issue 81 August 1993In this issue RelatedDetailsLoading ..." @default.
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• W1446286296 date "1993-08-01" @default.
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• W1446286296 title "Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96." @default.
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• W1446286296 doi "https://doi.org/10.1002/j.1460-2075.1993.tb05975.x" @default.
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