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- W1447343824 abstract "Publisher Summary In situ hybridization offers the ability to (a) examine regional changes in mRNA expression in a tissue, (b) determine the cell type expressing a specific mRNA, and (c) locate a specific mRNA in the cell. Because of aforementioned properties in situ hybridization is a powerful complement to blotting techniques as well as morphological techniques for studies of gene expression and development. This chapter describes the in situ hybridization of retinal messenger RNA using nonradioactive synthetic probes. The in situ hybridization provides the ability to monitor changes in the quantity of a particular mRNA in individual cells in the context of small areas of the retina. This ability to examine small numbers of cells is expected to be of a particular value in understanding retinal development. It has been found that regional changes in normal or transgene expression in different classes of retinal cells are better visualized by direct hybridization of DNA or RNA probes to tissue sections." @default.
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- W1447343824 date "1993-01-01" @default.
- W1447343824 modified "2023-09-24" @default.
- W1447343824 title "In Situ Hybridization of Retinal Messenger RNA Using Nonradioactive Synthetic Probes" @default.
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- W1447343824 doi "https://doi.org/10.1016/b978-0-12-185279-5.50032-7" @default.
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