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- W1449441462 abstract "Primary structure is the starting point for all modeling studies and the chapter summarizes the most current methods of computer modeling of the combining site structure of anti-hapten monoclonal antibodies. Because of major advances in the synthesis of oligonucleotides of predefined sequence, the determination of immunoglobulin variable region in amino acid sequence via gene sequencing is now straightforward. Several approaches are currently being employed. The most direct method involves sequencing heavy- and light-chain immunoglobulin mitochondrial RNA (mRNA) isolated from hybridoma cells. Oligonucleotides that specifically bind to conserved sequences near the variable-constant region junction of immunoglobulin mRNA are extended in the presence of dideoxynucleotides using reverse transcriptase to generate the sequencing ladder. Rapidly expanding primary sequence data from molecular biology and polymerase chain reaction (PCR) technology have provided a vast source of potential input data for the generation of three-dimensional models. The process of computer model building has the potential to add to knowledge of the structural basis of anti-idiotype recognition and to aid the process of designing therapeutic agents in autoimmune disease. Due to the complexity and expense, very few antibodies that are used in clinical assays have been rationally engineered for enhanced affinity and specificity. Antibody models may be used to guide the process of protein engineering to produce exquisite selectivity in anti-drug antibodies." @default.
- W1449441462 created "2016-06-24" @default.
- W1449441462 creator A5014673831 @default.
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- W1449441462 date "1991-01-01" @default.
- W1449441462 modified "2023-10-15" @default.
- W1449441462 title "[2] Computer modeling of combining site structure of anti-hapten monoclonal antibodies" @default.
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- W1449441462 doi "https://doi.org/10.1016/0076-6879(91)03004-z" @default.
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