FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W144967692> ?p ?o ?g. }

• W144967692 endingPage "27" @default.
• W144967692 startingPage "16" @default.
• W144967692 abstract "During the last two decades, significant advances have been made in the fields of lactococcal genetics and protein expression. Lactococcus lactis (L. lactis) is an effective vector for protein expression and can be used as an antigen delivery system. Hence, L. lactis is an ideal candidate for mucosal immunotherapy. Profilin (Che a 2), the major allergen in Chenopodium album, is one of the most important causes of allergic diseases in desert and semi-desert areas, especially in Iran, Saudi Arabia, and Kuwait that was cloned and expressed in L. lactis for the first time.To construct L. lactis that expressed Che a 2, a DNA sequence was cloned and used to transform bacteria. Expression of Che a 2 was analyzed via monitoring of related RNA and protein. Hydrophobicity, adherence to HT-29 cells, antibiotic resistance, resistance to gastrointestinal contents, pH, and bile salt in recombinant and native L. lactis were evaluated.Immunoblot analyses demonstrated that recombinant Che a 2 is expressed as a 32 kDa dimeric protein immunological studies showed it can bind human IgE. Both native and recombinant bacteria were sensitive to low pH and simulated gastric conditions. Bacterial survival was reduced 80-100% after 2 h of exposure to pH 1.5-2. Both native and recombinant bacteria were able to grow in 0.3 and 2% bile salts. After incubation of recombinant L. lactis in simulated gastric and intestinal juices for one and two hours, respectively, cell survival was reduced by 100%. Adhesion capability in both strains was minimal and there were no significant differences in any of our tests between native and recombinant bacteria.Successfully recombinant L. lactis with capability of expression Che a 2 was produced and revealed it is sensitive to gastrointestinal contents." @default.
• W144967692 created "2016-06-24" @default.
• W144967692 creator A5002259570 @default.
• W144967692 creator A5009207901 @default.
• W144967692 creator A5025288541 @default.
• W144967692 creator A5060342699 @default.
• W144967692 creator A5070835504 @default.
• W144967692 date "2013-10-01" @default.
• W144967692 modified "2023-09-27" @default.
• W144967692 title "Construction of a recombinant allergen-producing probiotic bacterial strain: Introduction of a new line for a live oral vaccine against Chenopodium album pollen allergy." @default.
• W144967692 cites W1566957489 @default.
• W144967692 cites W1621450368 @default.
• W144967692 cites W1829239725 @default.
• W144967692 cites W1975165965 @default.
• W144967692 cites W1976316617 @default.
• W144967692 cites W1978358524 @default.
• W144967692 cites W1978919933 @default.
• W144967692 cites W1981714645 @default.
• W144967692 cites W1982273763 @default.
• W144967692 cites W1992019228 @default.
• W144967692 cites W1992153121 @default.
• W144967692 cites W1998818706 @default.
• W144967692 cites W2002703938 @default.
• W144967692 cites W2006896654 @default.
• W144967692 cites W2008898532 @default.
• W144967692 cites W2013103459 @default.
• W144967692 cites W2032111794 @default.
• W144967692 cites W2034030958 @default.
• W144967692 cites W2037476918 @default.
• W144967692 cites W2042381017 @default.
• W144967692 cites W2042931688 @default.
• W144967692 cites W2046326849 @default.
• W144967692 cites W2053181580 @default.
• W144967692 cites W2056136884 @default.
• W144967692 cites W2057315622 @default.
• W144967692 cites W2066643327 @default.
• W144967692 cites W2080859586 @default.
• W144967692 cites W2080953466 @default.
• W144967692 cites W2089099400 @default.
• W144967692 cites W2091505261 @default.
• W144967692 cites W2091587044 @default.
• W144967692 cites W2097731012 @default.
• W144967692 cites W2107787922 @default.
• W144967692 cites W2109218969 @default.
• W144967692 cites W2113645094 @default.
• W144967692 cites W2127092918 @default.
• W144967692 cites W2130614990 @default.
• W144967692 cites W2132644314 @default.
• W144967692 cites W2139906115 @default.
• W144967692 cites W2149774675 @default.
• W144967692 cites W2157254889 @default.
• W144967692 cites W2161440397 @default.
• W144967692 cites W2162505448 @default.
• W144967692 cites W2163292218 @default.
• W144967692 cites W2166955843 @default.
• W144967692 cites W2167113011 @default.
• W144967692 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4757063" @default.
• W144967692 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26989716" @default.
• W144967692 hasPublicationYear "2013" @default.
• W144967692 type Work @default.
• W144967692 sameAs 144967692 @default.
• W144967692 citedByCount "0" @default.
• W144967692 crossrefType "journal-article" @default.
• W144967692 hasAuthorship W144967692A5002259570 @default.
• W144967692 hasAuthorship W144967692A5009207901 @default.
• W144967692 hasAuthorship W144967692A5025288541 @default.
• W144967692 hasAuthorship W144967692A5060342699 @default.
• W144967692 hasAuthorship W144967692A5070835504 @default.
• W144967692 hasConcept C104317684 @default.
• W144967692 hasConcept C159047783 @default.
• W144967692 hasConcept C2775920511 @default.
• W144967692 hasConcept C2780011460 @default.
• W144967692 hasConcept C40767141 @default.
• W144967692 hasConcept C523546767 @default.
• W144967692 hasConcept C54355233 @default.
• W144967692 hasConcept C55493867 @default.
• W144967692 hasConcept C86803240 @default.
• W144967692 hasConcept C89423630 @default.
• W144967692 hasConceptScore W144967692C104317684 @default.
• W144967692 hasConceptScore W144967692C159047783 @default.
• W144967692 hasConceptScore W144967692C2775920511 @default.
• W144967692 hasConceptScore W144967692C2780011460 @default.
• W144967692 hasConceptScore W144967692C40767141 @default.
• W144967692 hasConceptScore W144967692C523546767 @default.
• W144967692 hasConceptScore W144967692C54355233 @default.
• W144967692 hasConceptScore W144967692C55493867 @default.
• W144967692 hasConceptScore W144967692C86803240 @default.
• W144967692 hasConceptScore W144967692C89423630 @default.
• W144967692 hasIssue "1" @default.
• W144967692 hasLocation W1449676921 @default.
• W144967692 hasLocation W1449676922 @default.
• W144967692 hasOpenAccess W144967692 @default.
• W144967692 hasPrimaryLocation W1449676921 @default.
• W144967692 hasRelatedWork W1487857142 @default.
• W144967692 hasRelatedWork W1557899908 @default.
• W144967692 hasRelatedWork W1624964834 @default.
• W144967692 hasRelatedWork W1782535921 @default.
• W144967692 hasRelatedWork W2088745698 @default.

• next