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- W144990088 abstract "Supercritical fluid extraction (SFE) is an attractive alternative technique toconventional liquid extraction due to its several distinct properties. This novelinteresting extraction method which was developed in 1960 is an energy efficient,economically viable and environmentally friendly process. This study evaluated theeffect of supercritical carbon dioxide extraction on the extraction yields and majorbioactive flavonoid compounds from the herbal matrices. Two basic extractionmethods were investigated: conventional soxhlet extraction (CSE) and supercriticalcarbon dioxide (SC-CO2) extraction. High-Performance Liquid Chromatography(HPLC) was used to identify and quantify bioactive flavonoid compounds ofproduced extracts. Results obtained from the two extraction methods were comparedfor a higher extraction yield and concentration of flavonoid compounds. In the studyof supercritical carbon dioxide extraction and conventional soxhlet extractionspearmint (Mentha spicata L.) leaves were selected. For optimizing of SC-CO2 extraction process three most important variables including temperature, pressureand extraction dynamic time have been studied. The full factorial in completerandomize design (CRD) based on three levels and three factors was employed toobtain the optimum condition for SFE. Based on the simultaneous optimization ofcrude extract yield and concentration of flavonoid compounds the optimumcondition was found at temperature of 60 ᵒC, pressure of 200 bar and extractiondynamic time of 60 min. In conventional soxhlet extraction study, different solventswere used to evaluate the effect of different applied solvents on the extraction yieldand major bioactive flavonoid compounds. Ethanol: water (70:30) was found as apreferable solvent among the other applied solvents due to its higher extraction yield,flavonoid compounds concentration and lower toxicity effects. Compared withsupercritical carbon dioxide extraction the higher concentration of bioactiveflavonoid compounds was obtained and extraction time was reduced by applyingSC-CO2 extraction. However, the higher crude extract yields were obtained by usingconventional soxhlet extraction. The influence of co-solvent (modifier) on theextraction yield and extracted flavonoid compounds from spearmint (Mentha spicataL.) leaves was also studied. In this study ethanol acted as co-solvent to improve theefficiency of polar compounds (flavonoids) extraction. Co-solvent flow rate of 6g/min was found as a preferable modifier flow rate to obtain higher extraction yieldand bioactive flavonoid compounds concentration." @default.
- W144990088 created "2016-06-24" @default.
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- W144990088 date "2009-01-01" @default.
- W144990088 modified "2023-09-24" @default.
- W144990088 title "Supercritical Fluid Extraction of Major Bioactive Flavonoids from Spearmint (Mentha Spicata L.) Leaves" @default.
- W144990088 hasPublicationYear "2009" @default.
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