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• W1451958334 abstract "Digoxigenin-labelled total, Leishmania donovani DNA was used as a probe to detect the parasite in Indian Phlebotomus argentipes. The probe was quite sensitive, detecting as little as 0.3 pg parasite DNA, equivalent to approximately 100 parasites. Positive signals could be detected in 12 (32%) of the 38 small (two- to 30-fly) pools of the wild-caught P. argentipes investigated and in a pool of 10 laboratory-bred P. argentipes fed on a patient with post-kala-azar dermal leishmaniasis. None of the wild-caught P. papatasi, P. sergenti or Sergentomyia sp. or of the newly emerged, laboratory-bred P. argentipes investigated were positive by this probe. The results indicate that such a probe may be very useful in screening sandflies for L. donovani, and so detecting the main vectors and transmission pathways of this parasite, in India and elsewhere." @default.
• W1451958334 created "2016-06-24" @default.
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• W1451958334 date "2000-07-01" @default.
• W1451958334 modified "2023-09-30" @default.
• W1451958334 title "Screening sandflies for natural infection withLeishmania donovani, using a non-radioactive probe based on the total DNA of the parasite" @default.
• W1451958334 cites W2066826381 @default.
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• W1451958334 doi "https://doi.org/10.1080/00034983.2000.11813563" @default.
• W1451958334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10983557" @default.
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