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- W1454998416 abstract "We have developed a method for the T4 DNA ligase-catalyzed DNA-templated polymerization of 5′-phosphorylated pentanucleotides containing peptide fragments. The polymerization proceeds sequence-specifically to generate DNA-scaffolded peptides in excellent yields. The method has been shown to tolerate peptides ranging from two to eight amino acids in length with a wide variety of functionality. We validated the capabilities of this system in a mock selection for the enrichment of a His-tagged DNA-scaffolded peptide phenotype from a library, which exhibited a 190-fold enrichment after one round of selection. This strategy demonstrates a promising new approach to allowing the generation and in vitro selection of high-affinity reagents based upon single-stranded DNA scaffolding of peptide fragments." @default.
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- W1454998416 date "2015-08-21" @default.
- W1454998416 modified "2023-09-27" @default.
- W1454998416 title "Sequence-Defined Scaffolding of Peptides on Nucleic Acid Polymers" @default.
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- W1454998416 doi "https://doi.org/10.1021/jacs.5b07675" @default.
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