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• W1456210008 abstract "Research Article1 April 1987free access Characterization of the virA locus of Agrobacterium tumefaciens: a transcriptional regulator and host range determinant. B. Leroux B. Leroux Search for more papers by this author M.F. Yanofsky M.F. Yanofsky Search for more papers by this author S.C. Winans S.C. Winans Search for more papers by this author J.E. Ward J.E. Ward Search for more papers by this author S.F. Ziegler S.F. Ziegler Search for more papers by this author E.W. Nester E.W. Nester Search for more papers by this author B. Leroux B. Leroux Search for more papers by this author M.F. Yanofsky M.F. Yanofsky Search for more papers by this author S.C. Winans S.C. Winans Search for more papers by this author J.E. Ward J.E. Ward Search for more papers by this author S.F. Ziegler S.F. Ziegler Search for more papers by this author E.W. Nester E.W. Nester Search for more papers by this author Author Information B. Leroux, M.F. Yanofsky, S.C. Winans, J.E. Ward, S.F. Ziegler and E.W. Nester The EMBO Journal (1987)6:849-856https://doi.org/10.1002/j.1460-2075.1987.tb04830.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The virulence (vir) region of Agrobacterium tumefaciens mediates the transfer of a defined segment of plasmid DNA (the T-DNA) into the plant genome. The vir genes are specifically induced by molecules produced by wounded plant cells, and virA is required for this induction. We have determined the nucleotide sequence of virA loci from limited (pTiAg162) and wide (pTiA6) host range tumor-inducing (Ti) plasmids, each of which encodes a single protein of 92,000 daltons. Using antibody directed against the virA gene product, we have localized the VirA protein to the bacterial inner membrane. VirA is homologous to at least four bacterial proteins which play a role in the transcriptional regulation of diverse families of genes. Based on its role in vir gene induction, homology to transcriptional regulators and membrane localization, we propose that VirA acts as an environmental sensor of plant-derived inducer molecules and transmits this information to the level of vir gene expression. The pTiAg162 virA locus was shown to be ineffective at directing vir gene induction, suggesting that this may in part contribute to the narrow host range conferred by this plasmid. Previous ArticleNext Article Volume 6Issue 41 April 1987In this issue RelatedDetailsLoading ..." @default.
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• W1456210008 date "1987-04-01" @default.
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• W1456210008 title "Characterization of the virA locus of Agrobacterium tumefaciens: a transcriptional regulator and host range determinant." @default.
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• W1456210008 doi "https://doi.org/10.1002/j.1460-2075.1987.tb04830.x" @default.
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