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- W1456484142 abstract "Publisher Summary This chapter reviews some of the procedures that have been used for the isolation and characterization of nascent lipoproteins from the subcellular organelles involved in plasma protein secretion obtained from liver. The emphasis is on techniques that have been useful in studies of high-density lipoproteins (HDL) synthesis and secretion by the liver of young chickens. In vivo studies, in which radiolabeled precursors of the protein and/or lipid moieties are used, are valuable in determining the types of apolipoproteins and/or lipids made by the liver. One of the major limitations of the in vivo labeling technique is obtaining sufficient incorporation of radioactive precursors into the isolated nascent lipoproteins. Despite the limitations which have been enumerated, the combination of kinetic studies, cell fractionation, and electron microscopy is an effective method in eliciting information of the intracellular sequence of events which leads to the formation of nascent lipoproteins." @default.
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- W1456484142 date "1986-01-01" @default.
- W1456484142 modified "2023-09-24" @default.
- W1456484142 title "Studies of nascent lipoproteins in isolated hepatic microsomes and Golgi cell fractions" @default.
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- W1456484142 doi "https://doi.org/10.1016/0076-6879(86)29076-x" @default.
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