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- W145657185 abstract "This chapter discusses the reduced binding of ouabain by chronic lymphocytic leukemia lymphocytes. In a study described in the chapter, lymphocytes were separated from fresh heparinized human peripheral blood using Ficoll–Hypaque and were cultured in microtitration plates in 0.1 ml RPMI medium, buffered with 10 mM Hepes, at a cellular content of 105 per well. Phytohemagglutinin was used to stimulate the cells, DNA being measured by the incorporation of H3-thymidine. Ouabain binding to the lymphocytes at various concentrations was assessed using the tritiated glycoside. The uptake of K42 and Rb86 was measured at various intervals after administration of the isotopes to the medium surrounding the cells, using an automated cell harvester. Enrichment of the T and B lymphocyte subpopulations was carried out by the method of Gmelig–Meyling and Ballieux. The uptake of H3-ouabain and active transport of K42 and Rb86 by human lymphocytes, corresponding to different concentrations of ouabain in the culture medium, is illustrated in the chapter. Nearly mirror plots were observed for ouabain binding and the corresponding uptake of monovalent cation." @default.
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- W145657185 date "1979-01-01" @default.
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- W145657185 title "REDUCED BINDING OF OUABAIN BY CHRONIC LYMPHOCYTIC LEUKEMIA LYMPHOCYTES" @default.
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- W145657185 doi "https://doi.org/10.1016/b978-0-12-569650-0.50042-5" @default.
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