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- W1458298451 abstract "This chapter describes the preparation of subunits of bacterial luciferase, which is a heterodimer designated αβ. Results of luciferase chemical and mutational modifications show that α is the catalytic subunit, α and β subunits are not active individually; the specific function of β is however not clear. The interaction affinity between the two subunits is apparently high, so the isolation of individual luciferase subunits by ion-exchange column chromatography requires the presence of a denaturant. After a single step of column chromatography, α and β subunits can be well separated with good reproducibilities. The inactive protein species in the isolated α and β samples do not seem to perturb the activity of a diluted native luciferase sample. Procedures for the purification of luciferase from cells of Beneckea harveyi and the standard-assay method for the measurement of luciferase activity are both described in the chapter." @default.
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- W1458298451 date "1978-01-01" @default.
- W1458298451 modified "2023-10-17" @default.
- W1458298451 title "[17] Preparation of the subunits of bacterial luciferase" @default.
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- W1458298451 doi "https://doi.org/10.1016/0076-6879(78)57019-5" @default.
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