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- W1459690006 abstract "This chapter discusses the use of 125I-labeled anti-2,4-dinitrophenyl (DNP) antibodies as a general tracer in solid-phase radioimmunoassays (RIA). The need to use distinct immunochemically purified and radiolabeled antibodies for each RIA may be obviated, by using instead haptenated immunoglobulins, that can be quantitated, by measuring the subsequent attachment of purified radiolabeled antihapten antibodies to antigen–antibody complexes. Such radiolabeled antibodies can be utilized as a general tracer in solid-phase RIA. The principle of the method, for which the 2,4-dinitrophenyl (DNP) group was selected as hapten, is discussed in the chapter. Immunoglobulins (IgG) used for coating of polystyrene beads and for labeling with DNP are prepared from antisera by chromatography on diethylaminoethyl cellulose (DEAE)-cellulose. The average number of 2,4-dinitrophenol (DNP) groups incorporated per IgG molecule, determined from spectrophotometric measurements, using DNP-lysine as a standard, increases with the concentration of 2,4-dinitro-benzene sulphonate (DNBS). Supraoptimal haptenation leads to nonspecific binding of DNP antibodies to the solid phase, and consequently to the diminished sensitivity in RIA tests. RIA has the advantages that include the use of a single radiolabeled antibody in all tests and introduction of the DNP groups into the primary antibodies leads to amplified radioactive tracer binding." @default.
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- W1459690006 date "1981-01-01" @default.
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- W1459690006 title "[7] Use of 125I-labeled anti-2,4-dinitrophenyl (DNP) antibodies as a general tracer in solid-phase radioimmunoassays" @default.
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- W1459690006 doi "https://doi.org/10.1016/0076-6879(81)73060-x" @default.
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