Matches in SemOpenAlex for { <https://semopenalex.org/work/W1459791060> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1459791060 endingPage "320" @default.
- W1459791060 startingPage "311" @default.
- W1459791060 abstract "This chapter presents procedure for preparation of high molecular weight glycopeptides and oligosaccharides (erythroglycan) from human erythrocytes. Human erythrocyte membranes are prepared and delipidated by chloroform-methanol and water-n-butanol extraction. Oligosaccharides are released from this dry, lipid-free residue by hydrazinolysis, or the corresponding glycopeptides are prepared by exhaustive digestion with Pronase and cleavage of O-glycosidic bonds by mild alkaline borohydride treatment. The high molecular weight oligosaccharides or glycopeptides are separated from the smaller glycans by gel filtration giving a molecular weight profile of the total glycopeptides of the erythrocyte. The pooled fraction of glycopeptides of molecular weight 7000 to 12,000 constitutes the erythroglycan fraction. In an optional modification of this method, the cells or membranes are pretreated with trypsin, which removes most of the lower molecular weight protein-bound saccharide chains but none of the erythroglycan. In this modification the low molecular weight glycopeptides are removed prior to further processing by washing the trypsinized cells." @default.
- W1459791060 created "2016-06-24" @default.
- W1459791060 creator A5020530829 @default.
- W1459791060 creator A5025901599 @default.
- W1459791060 creator A5050173666 @default.
- W1459791060 creator A5055944397 @default.
- W1459791060 creator A5086766467 @default.
- W1459791060 date "1982-01-01" @default.
- W1459791060 modified "2023-10-16" @default.
- W1459791060 title "[24] Preparation of high molecular weight glycopeptides and oligosaccharides (erythroglycan) from Human erythrocytes" @default.
- W1459791060 cites W1504533936 @default.
- W1459791060 cites W1511913702 @default.
- W1459791060 cites W1593261391 @default.
- W1459791060 cites W1594682500 @default.
- W1459791060 cites W1612151898 @default.
- W1459791060 cites W1840072061 @default.
- W1459791060 cites W1951551989 @default.
- W1459791060 cites W1966594604 @default.
- W1459791060 cites W1967987315 @default.
- W1459791060 cites W1985957756 @default.
- W1459791060 cites W1992534906 @default.
- W1459791060 cites W2000648016 @default.
- W1459791060 cites W2013166956 @default.
- W1459791060 cites W2014329296 @default.
- W1459791060 cites W2021441668 @default.
- W1459791060 cites W2037947026 @default.
- W1459791060 cites W2047009326 @default.
- W1459791060 cites W2052664734 @default.
- W1459791060 cites W2056299536 @default.
- W1459791060 cites W2068586690 @default.
- W1459791060 cites W2088049062 @default.
- W1459791060 cites W2089990975 @default.
- W1459791060 cites W2090249202 @default.
- W1459791060 cites W2095358418 @default.
- W1459791060 cites W2099166024 @default.
- W1459791060 cites W2183588926 @default.
- W1459791060 cites W2289489971 @default.
- W1459791060 doi "https://doi.org/10.1016/0076-6879(82)83026-7" @default.
- W1459791060 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7115541" @default.
- W1459791060 hasPublicationYear "1982" @default.
- W1459791060 type Work @default.
- W1459791060 sameAs 1459791060 @default.
- W1459791060 citedByCount "7" @default.
- W1459791060 countsByYear W14597910602017 @default.
- W1459791060 crossrefType "book-chapter" @default.
- W1459791060 hasAuthorship W1459791060A5020530829 @default.
- W1459791060 hasAuthorship W1459791060A5025901599 @default.
- W1459791060 hasAuthorship W1459791060A5050173666 @default.
- W1459791060 hasAuthorship W1459791060A5055944397 @default.
- W1459791060 hasAuthorship W1459791060A5086766467 @default.
- W1459791060 hasConcept C108625454 @default.
- W1459791060 hasConcept C137610392 @default.
- W1459791060 hasConcept C181199279 @default.
- W1459791060 hasConcept C185592680 @default.
- W1459791060 hasConcept C193363816 @default.
- W1459791060 hasConcept C206212055 @default.
- W1459791060 hasConcept C2776708817 @default.
- W1459791060 hasConcept C2777726330 @default.
- W1459791060 hasConcept C2780340462 @default.
- W1459791060 hasConcept C2781338088 @default.
- W1459791060 hasConcept C38240268 @default.
- W1459791060 hasConcept C41625074 @default.
- W1459791060 hasConcept C43617362 @default.
- W1459791060 hasConcept C501593827 @default.
- W1459791060 hasConcept C55493867 @default.
- W1459791060 hasConceptScore W1459791060C108625454 @default.
- W1459791060 hasConceptScore W1459791060C137610392 @default.
- W1459791060 hasConceptScore W1459791060C181199279 @default.
- W1459791060 hasConceptScore W1459791060C185592680 @default.
- W1459791060 hasConceptScore W1459791060C193363816 @default.
- W1459791060 hasConceptScore W1459791060C206212055 @default.
- W1459791060 hasConceptScore W1459791060C2776708817 @default.
- W1459791060 hasConceptScore W1459791060C2777726330 @default.
- W1459791060 hasConceptScore W1459791060C2780340462 @default.
- W1459791060 hasConceptScore W1459791060C2781338088 @default.
- W1459791060 hasConceptScore W1459791060C38240268 @default.
- W1459791060 hasConceptScore W1459791060C41625074 @default.
- W1459791060 hasConceptScore W1459791060C43617362 @default.
- W1459791060 hasConceptScore W1459791060C501593827 @default.
- W1459791060 hasConceptScore W1459791060C55493867 @default.
- W1459791060 hasLocation W14597910601 @default.
- W1459791060 hasLocation W14597910602 @default.
- W1459791060 hasOpenAccess W1459791060 @default.
- W1459791060 hasPrimaryLocation W14597910601 @default.
- W1459791060 hasRelatedWork W1459791060 @default.
- W1459791060 hasRelatedWork W1603379725 @default.
- W1459791060 hasRelatedWork W1981905369 @default.
- W1459791060 hasRelatedWork W1992364866 @default.
- W1459791060 hasRelatedWork W1998342774 @default.
- W1459791060 hasRelatedWork W2003020379 @default.
- W1459791060 hasRelatedWork W2004239288 @default.
- W1459791060 hasRelatedWork W2031295458 @default.
- W1459791060 hasRelatedWork W2063591361 @default.
- W1459791060 hasRelatedWork W2073204417 @default.
- W1459791060 isParatext "false" @default.
- W1459791060 isRetracted "false" @default.
- W1459791060 magId "1459791060" @default.
- W1459791060 workType "book-chapter" @default.