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- W146069450 abstract "Protein–DNA interactions are critical to maintain genome stability, DNA replication, chromosome segregation and to regulate gene expression. Chromatin immunoprecipitation (ChIP) is a powerful technique to study these interactions within living neurons and nervous tissue. In particular, ChIP analysis of chromatin in which protein–DNA interactions are first fixed in situ provides a valuable approach to identify specific transcription factor–DNA interactions and their regulation in the developing nervous system. Here we describe a procedure utilizing Percoll gradient purification of nuclei from fresh brain tissue pre-fixed with formaldehyde for ChIP analysis. This purification protocol provides an enrichment of neuronal nuclei in high yield. We also illustrate the suitability of chromatin prepared from Percoll-purified brain nuclei for ChIP analysis of regulated transcription factor interactions with neuronal gene promoters." @default.
- W146069450 created "2016-06-24" @default.
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- W146069450 date "2013-01-01" @default.
- W146069450 modified "2023-10-03" @default.
- W146069450 title "Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei" @default.
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- W146069450 doi "https://doi.org/10.1007/978-1-62703-444-9_19" @default.
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