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- W1464499032 abstract "This chapter describes the large-scale isolation of unseparated bacterial chain elongation factors, Tu and Ts (jointly referred to as T) and of G, discusses a micromethod for the isolation of unseparated Tu, Ts, and G; and the isolation of Tu and Ts. The important considerations for successful large-scale purification of T and G factors are (1) attention to the growth conditions, and (2) the use of a fast, reproducible, batchwise step to separate the factors. The batchwise separation of the T and G factors is affected by a preliminary quantitative removal of nucleic acids using the polyethylene glycol-Dextran aqueous two-phase system, followed by ammonium sulfate precipitation and back elution of the precipitate with decreasing concentrations of ammonium sulfate. The batchwise separation has advantages, in that it permits almost unlimited scaling-up and eliminates the necessity for performing assays to localize the active fractions. Although, very little free Tu is found in the crude soluble fraction of the cell because, presumably, it will have interacted with free Ts to form T, Tu is present on slightly washed ribosomes." @default.
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- W1464499032 date "1971-01-01" @default.
- W1464499032 modified "2023-09-26" @default.
- W1464499032 title "[29] Isolation of bacterial chain elongation factors" @default.
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- W1464499032 doi "https://doi.org/10.1016/s0076-6879(71)20031-8" @default.
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