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• W1464724343 abstract "Research Article16 December 1985free access Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs. P.A. Krieg P.A. Krieg Search for more papers by this author D.A. Melton D.A. Melton Search for more papers by this author P.A. Krieg P.A. Krieg Search for more papers by this author D.A. Melton D.A. Melton Search for more papers by this author Author Information P.A. Krieg and D.A. Melton The EMBO Journal (1985)4:3463-3471https://doi.org/10.1002/j.1460-2075.1985.tb04105.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info To study the transcriptional regulation of genes during early Xenopus development, we have isolated a gene that is first transcribed at the mid-blastula transition. Transcription of this gene, called GS17, stops at mid-gastrula and the mRNA is rapidly degraded. Consequently, transcripts of GS17 are only present for a brief period, primarily during gastrulation. When the GS17 gene is injected into fertilized eggs, transcription from the injected DNA mimics the expression pattern of the endogenous gene, i.e., both the switch-on and switch-off of transcription are correctly regulated. The injected DNA is not significantly amplified and remains extrachromosomal. The correct expression of genes injected into Xenopus eggs will make it possible to investigate maternal factors involved in activating the embryonic genome. Previous ArticleNext Article Volume 4Issue 13A1 December 1985In this issue RelatedDetailsLoading ..." @default.
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• W1464724343 title "Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs." @default.
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• W1464724343 doi "https://doi.org/10.1002/j.1460-2075.1985.tb04105.x" @default.
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