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• W1464801841 startingPage "62" @default.
• W1464801841 abstract "Ex vivo lung perfusion (EVLP) has recently shown promise as a means of more accurately gauging the health of lung grafts and improving graft performance post-transplant. However, reperfusion of ischemic lung promotes the depletion of high-energy compounds and a progressive loss of normal mitochondrial function, and it remains unclear how and to what extent the EVLP approach contributes to this metabolic decline. Although ascorbate has been used to mitigate the effects of ischemia-reperfusion injury, the nature of its effects during EVLP are also not clear. To address these uncertainties, this study monitored the energy status of lungs during EVLP and after the administration of ascorbate using (31)P and hyperpolarized (13)C NMR (nuclear magnetic resonance). Our experiments demonstrated that the oxidative phosphorylation capacity and pyruvate dehydrogenase flux of lungs decline during ex vivo perfusion. The addition of ascorbate to the perfusate prolonged lung viability by 80% and increased the hyperpolarized (13)C bicarbonate signal by a factor of 2.7. The effect of ascorbate is apparently due not to its antioxidant quality but rather to its ability to energize cellular respiration given that it increased the lung's energy charge significantly, whereas other antioxidants (glutathione and α-lipoic acid) did not alter energy metabolism. During ascorbate administration, inhibition of mitochondrial complex I with rotenone depressed energy charge and shifted the metabolic state of the lung toward glycolysis; reenergizing the electron transport chain with TMPD (N,N,N',N'-tetramethyl-p-phenylenediamine) recovered metabolic activity. This indicates that ascorbate slows the decline of the ex vivo perfused lung's mitochondrial activity through an independent interaction with the electron transport chain complexes." @default.
• W1464801841 created "2016-06-24" @default.
• W1464801841 creator A5005347169 @default.
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• W1464801841 creator A5031512057 @default.
• W1464801841 date "2015-12-01" @default.
• W1464801841 modified "2023-10-17" @default.
• W1464801841 title "Ascorbic acid prolongs the viability and stability of isolated perfused lungs: A mechanistic study using 31P and hyperpolarized 13C nuclear magnetic resonance" @default.
• W1464801841 cites W1488213265 @default.
• W1464801841 cites W1504057237 @default.
• W1464801841 cites W1523213544 @default.
• W1464801841 cites W1530090249 @default.
• W1464801841 cites W1576683498 @default.
• W1464801841 cites W1701615504 @default.
• W1464801841 cites W181688216 @default.
• W1464801841 cites W1900634945 @default.
• W1464801841 cites W1912699316 @default.
• W1464801841 cites W1964319813 @default.
• W1464801841 cites W1965996434 @default.
• W1464801841 cites W1967790771 @default.
• W1464801841 cites W1970288522 @default.
• W1464801841 cites W1973487138 @default.
• W1464801841 cites W1974475788 @default.
• W1464801841 cites W1976133531 @default.
• W1464801841 cites W1976461058 @default.
• W1464801841 cites W1977823463 @default.
• W1464801841 cites W197864577 @default.
• W1464801841 cites W1981255631 @default.
• W1464801841 cites W1981907933 @default.
• W1464801841 cites W1982051678 @default.
• W1464801841 cites W1983688860 @default.
• W1464801841 cites W1983941658 @default.
• W1464801841 cites W1983967264 @default.
• W1464801841 cites W1988626276 @default.
• W1464801841 cites W1989904417 @default.
• W1464801841 cites W1990981872 @default.
• W1464801841 cites W1991113353 @default.
• W1464801841 cites W1992774368 @default.
• W1464801841 cites W1995484163 @default.
• W1464801841 cites W1997667793 @default.
• W1464801841 cites W2012645913 @default.
• W1464801841 cites W2012760268 @default.
• W1464801841 cites W201398293 @default.
• W1464801841 cites W2015640703 @default.
• W1464801841 cites W2018306907 @default.
• W1464801841 cites W2020770681 @default.
• W1464801841 cites W2028479043 @default.
• W1464801841 cites W2034353198 @default.
• W1464801841 cites W2037576801 @default.
• W1464801841 cites W2039131669 @default.
• W1464801841 cites W2045410354 @default.
• W1464801841 cites W2048723286 @default.
• W1464801841 cites W2049662677 @default.
• W1464801841 cites W2050164421 @default.
• W1464801841 cites W2058618870 @default.
• W1464801841 cites W2067204032 @default.
• W1464801841 cites W2070689041 @default.
• W1464801841 cites W2074030075 @default.
• W1464801841 cites W2075623551 @default.
• W1464801841 cites W2079090273 @default.
• W1464801841 cites W2079170185 @default.
• W1464801841 cites W2085115437 @default.
• W1464801841 cites W2085237138 @default.
• W1464801841 cites W2085268437 @default.
• W1464801841 cites W2086714169 @default.
• W1464801841 cites W2089090834 @default.
• W1464801841 cites W2091433029 @default.
• W1464801841 cites W2094742862 @default.
• W1464801841 cites W2095423676 @default.
• W1464801841 cites W2096494856 @default.
• W1464801841 cites W2101066016 @default.
• W1464801841 cites W2101840868 @default.
• W1464801841 cites W2102817107 @default.
• W1464801841 cites W2108227374 @default.
• W1464801841 cites W2109963570 @default.
• W1464801841 cites W2116890342 @default.
• W1464801841 cites W2127140678 @default.
• W1464801841 cites W2132826785 @default.
• W1464801841 cites W2141703874 @default.
• W1464801841 cites W2143127986 @default.
• W1464801841 cites W2154419874 @default.
• W1464801841 cites W2155111071 @default.
• W1464801841 cites W2160068784 @default.
• W1464801841 cites W2161411193 @default.
• W1464801841 cites W2162826918 @default.
• W1464801841 cites W2167527394 @default.
• W1464801841 cites W2275512643 @default.
• W1464801841 cites W2279946601 @default.
• W1464801841 cites W2314337161 @default.
• W1464801841 cites W2328198176 @default.
• W1464801841 cites W2344902841 @default.
• W1464801841 cites W4231680944 @default.
• W1464801841 cites W4241821292 @default.

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