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• W1466102795 endingPage "86" @default.
• W1466102795 startingPage "275" @default.
• W1466102795 abstract "Suspensions of enzymatically disaggregated hamster kidney cells were separated primarily according to differences in diameter using velocity sedimentation in a previously described isokinetic density gradient and according to differences in density in an isopycnic density gradient. Cells which contained histochemically demonstrable glucose-6-phosphatase were thought to be cells from proximal tubules and constituted 46.5 +/- 14.1% of the cells in the starting sample suspension of disaggregated kidney cells. The purest gradient fractions from experiments using velocity sedimentation contained 98.0 +/- 0.6% cells which demonstrated glucose-6-phosphatase activity. More than 99.0% of these cells excluded trypan blue. Isopycnic sedimentation was not an effective means of purifying proximal tubule cells." @default.
• W1466102795 created "2016-06-24" @default.
• W1466102795 creator A5020353737 @default.
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• W1466102795 date "1974-02-01" @default.
• W1466102795 modified "2023-09-23" @default.
• W1466102795 title "Separation of cells having histochemically demonstrable glucose-6-phosphatase from suspensions of hamster kidney cells in an isokinetic density gradient of Ficoll in tissue culture medium." @default.
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• W1466102795 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1910781" @default.
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• W1466102795 hasPublicationYear "1974" @default.
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