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- W1466701752 endingPage "72" @default.
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- W1466701752 abstract "This chapter covers advances made in the high-pressure or high-performance liquid chromatography (HPLC) of carbohydrates. In the chapter, the term “liquid chromatography (l.c.)” is used to designate the rapid (<1 h) separation of carbohydrates in modern, liquid-chromatography systems, which include a high-performance, solvent-delivery system using one or more pumps, an injection valve, a column packed with 3–10-μm, monodisperse, resin- or silica gel-based particles, and a high-performance detection system. These systems are operated at elevated pressures that, depending on the various stationary and mobile phases, the size of the columns employed, and the flow-rates used, may range from ∼1 to >30 MPa.This chapter discusses the 1.c. separations of almost all mono-, di-, and oligosaccharides from both a theoretical and a practical point of view. In addition, the general principles involved in the care and maintenance of the relevant instrumentation and stationary phases are covered and a separate section on preparative 1.c. methods for carbohydrates is included. The chapter also discusses solutions to the problems of detectability and of peak broadening (resolution of anomeric forms). It also considers the emerging 1.c. techniques, such as liquid chromatography-mass spectrometry (1.c.-m.s.) and high-performance affinity chromatography." @default.
- W1466701752 created "2016-06-24" @default.
- W1466701752 creator A5088900328 @default.
- W1466701752 date "1988-01-01" @default.
- W1466701752 modified "2023-10-13" @default.
- W1466701752 title "High-Performance Liquid Chromatography of Carbohydrates" @default.
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