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- W1466736110 abstract "This chapter discusses the clinical aspects of phosphohexose isomerase (PHI). Elevations of this enzyme activity have been found in the serum of patients with cancer, hepatitis, myocardial infarction various myopathies, leukemia, and other diseases. Fructose reacts in solution with resorcinol and HCl at a temperature of about 80° to form a red-colored compound. This is the well known Seliwanoff reaction for ketose sugars and consists in the formation, first, of hydroxymethylfurfural and, secondly, in the condensation of this compound with resorcinol to form a cherry red colored product or products. Heating under the usual conditions of this reaction, 15 minutes at 80° in 6N HCl, results in the hydrolysis of F-6-P to fructose and inorganic phosphate to the extent of 40–50%. Fructose and the unhydrolyzed F-6-P, reacts to the same extent with HCl and resorcinol on the basis of the fructose content. The method for PHI described is based on the conversion of G-6-P to F-6-P at pH 7.4 and 37°. The activity is defined as milliunits—that is, the millimicromoles of F-6-P formed per minute per milliliter of reaction mixture by 1 ml of serum or other biological fluid under the conditions described above. The calibration curve would ordinarily yield a value for the amount of F-6-P or fructose formed per milliliter of reaction mixture by 0.04 ml of serum in 30 minutes. The calibration curve may also be prepared so as to translate the absorbance readings directly into mill/units of activity by multiplying the F-6-P values on the abscissa by 1.00/0.04 × 1/30 or 0.833." @default.
- W1466736110 created "2016-06-24" @default.
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- W1466736110 date "1966-01-01" @default.
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- W1466736110 title "[99] Phosphohexose isomerase: Clinical aspects" @default.
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- W1466736110 doi "https://doi.org/10.1016/0076-6879(66)09113-4" @default.
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