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- W146775008 abstract "A previous study showed that anAutographa californicamulticapsid nuclear polyhedrosis virus (AcMNPV)25Kmutant produced less polyhedrin protein than wild-type (Jarviset al., J. Virol.66, 6903–6911, 1992). In this study, the role of the25Kgene product (AcMNPV ORF 61) in baculovirus gene expression was further investigated. Five different viral25Kmutants expressed lower levels of polyhedrin protein and less CAT activity under the control of thepolhpromoter compared to wild-type.PolhRNA was equally stable in wild-type and mutant virus-infected cells while the rate ofpolhtranscription was significantly reduced in mutant-infected cells. In comparison, steady-state levels ofp10RNA were not reduced in25Kmutant-infected cells, indicating that the reduction inpolhRNA did not reflect a general effect on very late gene transcription. Expression ofie-1,which also appears to influencepolhexpression (Choi and Guarino,Virology209, 90–98, 1995), was not influenced by25Kmutation. These results show that the 25K protein is important for maintaining optimal levels ofpolhtranscription by a mechanism that does not involve maintainingie-1expression." @default.
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- W146775008 date "1996-12-01" @default.
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- W146775008 title "The Role of the AcMNPV25KGene, “FP25,” in Baculoviruspolhandp10Expression" @default.
- W146775008 doi "https://doi.org/10.1006/viro.1996.0625" @default.
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