FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1468850792> ?p ?o ?g. }

• W1468850792 endingPage "323" @default.
• W1468850792 startingPage "297" @default.
• W1468850792 abstract "Focal adhesions (FAs) are discrete plasma membrane-associated adhesive organelles that play dual roles in cell force transduction and signaling. FAs consist of clustered transmembrane heterodimeric integrin extracellular matrix (ECM) receptors and a large number of cytoplasmic proteins that collectively form thin plaques linking the ECM to actin filament bundles of the cytoskeleton. FAs are complex organelles that can change their composition in response to biochemical or mechanical cues. These compositional differences may underlie the ability of FAs to mediate an array of important cell functions including adhesion, signaling, force transduction, and regulation of the cytoskeleton. These functions contribute to the physiological processes of the immune response, development, and differentiation. However, linking FA composition to FA function has been difficult since there has been no method to isolate intact FAs reproducibly and determine their composition. We report here a new method for isolating FA structures in cultured cells distinct from cytoplasmic, nuclear, and internal membranous organellar components of the cell. We provide protocols for validation of the fractionation by immunofluorescence and immunoblotting, procedures for preparing the isolated FAs for mass spectrometric proteomic analysis, tips on data interpretation and analysis, and an approach for comparing FA composition in cells in which small GTPase signaling is perturbed." @default.
• W1468850792 created "2016-06-24" @default.
• W1468850792 creator A5014863958 @default.
• W1468850792 creator A5044938342 @default.
• W1468850792 creator A5069076773 @default.
• W1468850792 creator A5074176340 @default.
• W1468850792 date "2011-01-01" @default.
• W1468850792 modified "2023-10-12" @default.
• W1468850792 title "Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis" @default.
• W1468850792 cites W1576773659 @default.
• W1468850792 cites W1597678601 @default.
• W1468850792 cites W1967265143 @default.
• W1468850792 cites W1990299806 @default.
• W1468850792 cites W1997504014 @default.
• W1468850792 cites W2003261719 @default.
• W1468850792 cites W2016605939 @default.
• W1468850792 cites W2019731805 @default.
• W1468850792 cites W2024367581 @default.
• W1468850792 cites W2026465178 @default.
• W1468850792 cites W2032601646 @default.
• W1468850792 cites W2054635074 @default.
• W1468850792 cites W2080553628 @default.
• W1468850792 cites W2091119718 @default.
• W1468850792 cites W2112093320 @default.
• W1468850792 cites W2112418598 @default.
• W1468850792 cites W2133874597 @default.
• W1468850792 cites W2135337307 @default.
• W1468850792 cites W2142501126 @default.
• W1468850792 cites W2152002380 @default.
• W1468850792 cites W2153132589 @default.
• W1468850792 doi "https://doi.org/10.1007/978-1-61779-166-6_19" @default.
• W1468850792 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4158431" @default.
• W1468850792 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21909920" @default.
• W1468850792 hasPublicationYear "2011" @default.
• W1468850792 type Work @default.
• W1468850792 sameAs 1468850792 @default.
• W1468850792 citedByCount "58" @default.
• W1468850792 countsByYear W14688507922012 @default.
• W1468850792 countsByYear W14688507922013 @default.
• W1468850792 countsByYear W14688507922014 @default.
• W1468850792 countsByYear W14688507922015 @default.
• W1468850792 countsByYear W14688507922016 @default.
• W1468850792 countsByYear W14688507922017 @default.
• W1468850792 countsByYear W14688507922018 @default.
• W1468850792 countsByYear W14688507922019 @default.
• W1468850792 countsByYear W14688507922020 @default.
• W1468850792 countsByYear W14688507922021 @default.
• W1468850792 countsByYear W14688507922022 @default.
• W1468850792 countsByYear W14688507922023 @default.
• W1468850792 crossrefType "book-chapter" @default.
• W1468850792 hasAuthorship W1468850792A5014863958 @default.
• W1468850792 hasAuthorship W1468850792A5044938342 @default.
• W1468850792 hasAuthorship W1468850792A5069076773 @default.
• W1468850792 hasAuthorship W1468850792A5074176340 @default.
• W1468850792 hasBestOaLocation W14688507922 @default.
• W1468850792 hasConcept C125705527 @default.
• W1468850792 hasConcept C142669718 @default.
• W1468850792 hasConcept C1491633281 @default.
• W1468850792 hasConcept C151166631 @default.
• W1468850792 hasConcept C168240541 @default.
• W1468850792 hasConcept C170493617 @default.
• W1468850792 hasConcept C185592680 @default.
• W1468850792 hasConcept C189165786 @default.
• W1468850792 hasConcept C190062978 @default.
• W1468850792 hasConcept C195687474 @default.
• W1468850792 hasConcept C24530287 @default.
• W1468850792 hasConcept C2993400109 @default.
• W1468850792 hasConcept C55493867 @default.
• W1468850792 hasConcept C62478195 @default.
• W1468850792 hasConcept C85789140 @default.
• W1468850792 hasConcept C86803240 @default.
• W1468850792 hasConcept C95444343 @default.
• W1468850792 hasConceptScore W1468850792C125705527 @default.
• W1468850792 hasConceptScore W1468850792C142669718 @default.
• W1468850792 hasConceptScore W1468850792C1491633281 @default.
• W1468850792 hasConceptScore W1468850792C151166631 @default.
• W1468850792 hasConceptScore W1468850792C168240541 @default.
• W1468850792 hasConceptScore W1468850792C170493617 @default.
• W1468850792 hasConceptScore W1468850792C185592680 @default.
• W1468850792 hasConceptScore W1468850792C189165786 @default.
• W1468850792 hasConceptScore W1468850792C190062978 @default.
• W1468850792 hasConceptScore W1468850792C195687474 @default.
• W1468850792 hasConceptScore W1468850792C24530287 @default.
• W1468850792 hasConceptScore W1468850792C2993400109 @default.
• W1468850792 hasConceptScore W1468850792C55493867 @default.
• W1468850792 hasConceptScore W1468850792C62478195 @default.
• W1468850792 hasConceptScore W1468850792C85789140 @default.
• W1468850792 hasConceptScore W1468850792C86803240 @default.
• W1468850792 hasConceptScore W1468850792C95444343 @default.
• W1468850792 hasLocation W14688507921 @default.
• W1468850792 hasLocation W14688507922 @default.
• W1468850792 hasLocation W14688507923 @default.
• W1468850792 hasLocation W14688507924 @default.
• W1468850792 hasOpenAccess W1468850792 @default.
• W1468850792 hasPrimaryLocation W14688507921 @default.
• W1468850792 hasRelatedWork W192658524 @default.
• W1468850792 hasRelatedWork W2002515131 @default.
• W1468850792 hasRelatedWork W2048357014 @default.

• next