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- W1469834900 abstract "Research Article1 December 1984free access Multiple non-allelic genes encoding pancreatic alpha-amylase of mouse are expressed in a strain-specific fashion. M. Tosi M. Tosi Search for more papers by this author R. Bovey R. Bovey Search for more papers by this author S. Astolfi S. Astolfi Search for more papers by this author S. Bodary S. Bodary Search for more papers by this author M. Meisler M. Meisler Search for more papers by this author P.K. Wellauer P.K. Wellauer Search for more papers by this author M. Tosi M. Tosi Search for more papers by this author R. Bovey R. Bovey Search for more papers by this author S. Astolfi S. Astolfi Search for more papers by this author S. Bodary S. Bodary Search for more papers by this author M. Meisler M. Meisler Search for more papers by this author P.K. Wellauer P.K. Wellauer Search for more papers by this author Author Information M. Tosi, R. Bovey, S. Astolfi, S. Bodary, M. Meisler and P.K. Wellauer The EMBO Journal (1984)3:2809-2816https://doi.org/10.1002/j.1460-2075.1984.tb02213.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The number of active Amy-2 genes has been estimated in strain CE/J mice which produce four distinct electrophoretic forms of alpha-amylase in their pancreas. cDNA cloning and DNA sequence analysis discloses five distinct mRNA sequences which differ by approximately 1% of their nucleotides. Two of these mRNAs specify the same protein. Changes in the nucleotide sequences result in amino acid replacements that alter the net charges of the deduced proteins. This has allowed a tentative assignment of individual mRNAs to isozymes detected by electrophoresis. Quantitative Southern blot hybridization using a DNA probe specific for the first exon of Amy-2 reveals the presence of greater than 10 Amy-2 related sequences per haploid CE/J genome. Models which could account for the mouse strain-specific differences with respect to the number of pancreatic alpha-amylase isozymes and their variable but genetically determined quantitative ratios are discussed. Previous ArticleNext Article Volume 3Issue 121 December 1984In this issue RelatedDetailsLoading ..." @default.
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- W1469834900 title "Multiple non-allelic genes encoding pancreatic alpha-amylase of mouse are expressed in a strain-specific fashion." @default.
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