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- W1471086676 abstract "This chapter describes the methods useful for obtaining 22P-labeled RNA transcription products that are derived from unique and limited regions of a DNA genome. Such RNA's reflect the sequence of the DNA from which they are transcribed and are suitable for hybridization procedures and for sequence analysis. Several approaches are presented. The first makes use of DNA restriction enzymes, such as those obtained from Hemophilus infiuenzae and H. aegyptius that are capable of cleaving DNA into small discrete products that can be purified by acrylamide gel electrophoresis. A second method makes use of DNA from deletionsubstitution mutants of viruses, to which transcribed RNA's may be annealed and from which RNA complementary to specific regions of the DNA, may be recovered and analyzed. The chapter describes procedures that have been developed in several laboratories. These techniques have been successfully employed to obtain RNA sequences for discrete regions of the DNA's of bacteriophage and SV40. The methodology presented provides a promising approach to the sequence analysis of large portions of the DNA of small viruses." @default.
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- W1471086676 date "1974-01-01" @default.
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- W1471086676 title "[22] Preparation of RNA transcripts of discrete segments of DNA" @default.
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- W1471086676 doi "https://doi.org/10.1016/0076-6879(74)29026-8" @default.
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