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- W1471225822 abstract "Publisher Summary This chapter highlights lymphocyte surface markers using alkaline phosphatase-labeled reagents. The results show that the relative proportions of lymphocytes expressing these markers are very constant; however, the absolute counts vary as the total lymphocyte count varies within its broad normal range. Similarly, in individuals studied repeatedly over periods of several months or years, the proportions of different lymphocyte populations remain constant but the absolute numbers vary as the subject's total lymphocyte count fluctuates. The method described in the chapter is effective and reproducible and does overcome the problems for accurate quantitation of lymphocyte populations posed by separation of mononuclear cells from blood before testing. It also offers possibilities for simultaneous examination of surface markers and of cytomorphology that may be valuable, particularly in leukemia. It has yielded information of physiological significance by providing normal values for adults and for well elderly subjects, and the capacity for enhanced sensitivity, which is characteristic of immunoenzyme methods, has confirmed the observations of others regarding the B-major and B-minor populations in normal individuals and extended these observations to patients with leukemia. A disadvantage of the method is the need to prepare special reagents, that is the alkaline phosphatase (AP) conjugates with F(ab´)2 antibodies and the immunopurified IgG and F(ab´)2 fragments of anti-AP serum. The chapter also presents the application of the enumeration of lymphocyte populations in whole peripheral blood." @default.
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- W1471225822 date "1983-01-01" @default.
- W1471225822 modified "2023-09-24" @default.
- W1471225822 title "[11] Demonstration of lymphocyte surface markers using alkaline phosphatase-labeled reagents. Application to the enumeration of lymphocyte populations in whole peripheral blood" @default.
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- W1471225822 doi "https://doi.org/10.1016/s0076-6879(83)93041-0" @default.
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