Matches in SemOpenAlex for { <https://semopenalex.org/work/W1471596317> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W1471596317 abstract "Fluorescent proteins of the Green Fluorescent Protein (GFP) family are important and versatile scientific tools used in a range of applications including the labelling of proteins, cells and tissue, high resolution imaging and studying protein-protein interactions. They exhibit a very diverse range of photochemical behaviour. Surprisingly this diversity originates from chromophores which have very similar structures. Accordingly, minor changes to the chromophore structure or to its surroundings in the protein can produce dramatic changes in optical behaviour.In this work the photophysics of the chromophores of the green fluorescent protein, the Kaede fluorescent protein and some related analogues are investigated using ultrafast methods; predominantly the excited state is probed using ultrafast fluorescence up-conversion with a time resolution of better than 50 fs. The excited states of chromophores decay on the ultrafast timescale. The decay will be probed for both isomers of the GFP chromophore, and the mechanism of decay will be modelled, leading to conclusions on the nature of the radiationless coordinate. The derivative of the chromophore generated in the Kaede protein will be studied, and marked differences with the GFP chromophore found.The importance of protein-chromophore interactions in influencing fluorescent protein behaviour will be demonstrated. The effects of chromophore modifications will be revealed through comparison of the analogues and the sensitivity of chromophore photophysics to the hydrogen bonding nature of the solvent will be demonstrated. It will also be shown that the Kaede chromophore undergoes aggregation enhanced fluorescence at relatively low concentrations." @default.
- W1471596317 created "2016-06-24" @default.
- W1471596317 creator A5076787934 @default.
- W1471596317 date "2015-03-01" @default.
- W1471596317 modified "2023-09-27" @default.
- W1471596317 title "The photophysics of fluorescent protein chromophores" @default.
- W1471596317 cites W1504770578 @default.
- W1471596317 cites W1551656287 @default.
- W1471596317 hasPublicationYear "2015" @default.
- W1471596317 type Work @default.
- W1471596317 sameAs 1471596317 @default.
- W1471596317 citedByCount "0" @default.
- W1471596317 crossrefType "dissertation" @default.
- W1471596317 hasAuthorship W1471596317A5076787934 @default.
- W1471596317 hasConcept C104317684 @default.
- W1471596317 hasConcept C120665830 @default.
- W1471596317 hasConcept C121332964 @default.
- W1471596317 hasConcept C142613039 @default.
- W1471596317 hasConcept C181500209 @default.
- W1471596317 hasConcept C185544564 @default.
- W1471596317 hasConcept C185592680 @default.
- W1471596317 hasConcept C192468462 @default.
- W1471596317 hasConcept C3019336641 @default.
- W1471596317 hasConcept C55493867 @default.
- W1471596317 hasConcept C75473681 @default.
- W1471596317 hasConcept C91881484 @default.
- W1471596317 hasConceptScore W1471596317C104317684 @default.
- W1471596317 hasConceptScore W1471596317C120665830 @default.
- W1471596317 hasConceptScore W1471596317C121332964 @default.
- W1471596317 hasConceptScore W1471596317C142613039 @default.
- W1471596317 hasConceptScore W1471596317C181500209 @default.
- W1471596317 hasConceptScore W1471596317C185544564 @default.
- W1471596317 hasConceptScore W1471596317C185592680 @default.
- W1471596317 hasConceptScore W1471596317C192468462 @default.
- W1471596317 hasConceptScore W1471596317C3019336641 @default.
- W1471596317 hasConceptScore W1471596317C55493867 @default.
- W1471596317 hasConceptScore W1471596317C75473681 @default.
- W1471596317 hasConceptScore W1471596317C91881484 @default.
- W1471596317 hasLocation W14715963171 @default.
- W1471596317 hasOpenAccess W1471596317 @default.
- W1471596317 hasPrimaryLocation W14715963171 @default.
- W1471596317 hasRelatedWork W124950745 @default.
- W1471596317 hasRelatedWork W1963548962 @default.
- W1471596317 hasRelatedWork W1989030054 @default.
- W1471596317 hasRelatedWork W1994955309 @default.
- W1471596317 hasRelatedWork W2000124531 @default.
- W1471596317 hasRelatedWork W2004304181 @default.
- W1471596317 hasRelatedWork W2008590376 @default.
- W1471596317 hasRelatedWork W2009643752 @default.
- W1471596317 hasRelatedWork W2012328359 @default.
- W1471596317 hasRelatedWork W2019716075 @default.
- W1471596317 hasRelatedWork W2024806163 @default.
- W1471596317 hasRelatedWork W2032961561 @default.
- W1471596317 hasRelatedWork W2045072358 @default.
- W1471596317 hasRelatedWork W2048770263 @default.
- W1471596317 hasRelatedWork W2049929644 @default.
- W1471596317 hasRelatedWork W2110466050 @default.
- W1471596317 hasRelatedWork W2152592178 @default.
- W1471596317 hasRelatedWork W2476543116 @default.
- W1471596317 hasRelatedWork W2580975438 @default.
- W1471596317 hasRelatedWork W2801401091 @default.
- W1471596317 isParatext "false" @default.
- W1471596317 isRetracted "false" @default.
- W1471596317 magId "1471596317" @default.
- W1471596317 workType "dissertation" @default.