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- W1472721622 abstract "Publisher Summary In recent years, a considerable number of monoclonal antibodies have been raised against epidermal growth factor (EGF) receptors. A table illustrated in this chapter shows EGF receptor monoclonal antibodies. A431 cells and A431 plasma membrane vesicles, as well as partially purified EGF receptors, have been successfully used to raise EGF receptor monoclonal antibodies. The chapter explains the production of EGF receptor monoclonal antibodies and discusses the screening of hybridomas and the characterization of EGF-receptor antibodies. The purification methods of monoclonal antibodies include protein A-agarose and DEAE-Affi-Gel Blue. As an intensively studied peptide growth factor, EGF induces several well-defined immediate and delayed responses in receptor-bearing target cells. The knowledge of the effects of EGF on responsive cells has made it possible to test EGF receptor monoclonal antibodies for agonistic and antagonistic biological activities." @default.
- W1472721622 created "2016-06-24" @default.
- W1472721622 creator A5006504847 @default.
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- W1472721622 date "1987-01-01" @default.
- W1472721622 modified "2023-10-17" @default.
- W1472721622 title "[6] Derivation and assay of biological effects of monoclonal antibodies to epidermal growth factor receptors" @default.
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- W1472721622 doi "https://doi.org/10.1016/s0076-6879(87)46009-6" @default.
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