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- W147333023 abstract "Fluorescence is a physical phenomenon in which a particular chemical compound (the fluorochrome) emits light of a particular color very shortly after being hit by light of another color. In this article we provide a brief survey of the physics of fluorescence and the design essentials of modern fluorescence microscopes. We further discuss in a practical sense fluorochromes and the importance of knowing their excitation and emission spectra. Appropriate filtering in single and multiple fluorescence applications is discussed as well, in addition to the problem of false positive results caused by fluorescence cross-talk." @default.
- W147333023 created "2016-06-24" @default.
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- W147333023 date "2009-01-01" @default.
- W147333023 modified "2023-10-18" @default.
- W147333023 title "Fluorescence Microscopy in the Neurosciences" @default.
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- W147333023 doi "https://doi.org/10.1016/b978-008045046-9.00666-5" @default.
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