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• W1473562009 abstract "Publisher Summary The chapter presents infrared absorption analysis of tissue constituents, particularly tissue lipids. The following types of biochemical applications of infrared spectroscopy have been distinguished: identification of pure compounds isolated by chromatography or other fractionation procedures; identification of compounds in mixtures; quantitative analysis of complex compounds that cannot be analyzed with chemical methods without difficulty or in the small samples available; determination of chemical structural features, such as isomeric structures; inference of bond directions in oriented material by measurement with polarized infrared radiation; and correlation of infrared spectra with biological properties. Problems concerning the clinical chemist or biochemist have been emphasized. The work on complex tissue constituents introduces two main problems: isolation of pure tissue constituents may be difficult and the yield may be too small to furnish a sufficiently large amount of material for complete chemical analysis. In other instances, the analysis may be carried out in complex mixtures of tissue material or with nonspecific methods for only a few constituent groups. It has been demonstrated in this chapter that chemical analysis can be effectively combined with chromatographic fractionation on columns, paper, and with gas-liquid chromatography, as well as with infrared spectroscopy, to overcome some of the difficulties arising in analysis of biologically important tissue components. Technical progress in infrared microanalysis by the introduction of the KBr disc technique has contributed considerably to the advance in this field. Other fractionation methods, such as countercurrent fractionation or reversed-phase chromatography may be included increasingly as the necessity arises. It is believed that these applications of infrared absorption spectroscopy in analysis of tissue constituents will become increasingly important to the clinical and biological chemist." @default.
• W1473562009 created "2016-06-24" @default.
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• W1473562009 date "1960-01-01" @default.
• W1473562009 modified "2023-10-18" @default.
• W1473562009 title "Infrared Absorption Analysis of Tissue Constituents, Particularly Tissue Lipids" @default.
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• W1473562009 doi "https://doi.org/10.1016/s0065-2423(08)60097-9" @default.
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