Matches in SemOpenAlex for { <https://semopenalex.org/work/W1473670137> ?p ?o ?g. }
- W1473670137 endingPage "23463" @default.
- W1473670137 startingPage "23447" @default.
- W1473670137 abstract "Dye-decolorizing peroxidases (DyPs) comprise a new family of heme peroxidases, which has received much attention due to their potential applications in lignin degradation. A new DyP from Thermomonospora curvata (TcDyP) was identified and characterized. Unlike other A-type enzymes, TcDyP is highly active toward a wide range of substrates including model lignin compounds, in which the catalytic efficiency with ABTS (kcatapp/Kmapp = (1.7 × 107) m−1 s−1) is close to that of fungal DyPs. Stopped-flow spectroscopy was employed to elucidate the transient intermediates as well as the catalytic cycle involving wild-type (wt) and mutant TcDyPs. Although residues Asp220 and Arg327 are found necessary for compound I formation, His312 is proposed to play roles in compound II reduction. Transient kinetics of hydroquinone (HQ) oxidation by wt-TcDyP showed that conversion of the compound II to resting state is a rate-limiting step, which will explain the contradictory observation made with the aspartate mutants of A-type DyPs. Moreover, replacement of His312 and Arg327 has significant effects on the oligomerization and redox potential (E°′) of the enzyme. Both mutants were found to promote the formation of dimeric state and to shift E°′ to a more negative potential. Not only do these results reveal the unique catalytic property of the A-type DyPs, but they will also facilitate the development of these enzymes as lignin degraders." @default.
- W1473670137 created "2016-06-24" @default.
- W1473670137 creator A5002389016 @default.
- W1473670137 creator A5042287156 @default.
- W1473670137 creator A5048246567 @default.
- W1473670137 creator A5056248574 @default.
- W1473670137 creator A5070640610 @default.
- W1473670137 creator A5073070877 @default.
- W1473670137 creator A5084307973 @default.
- W1473670137 creator A5084714769 @default.
- W1473670137 date "2015-09-01" @default.
- W1473670137 modified "2023-10-14" @default.
- W1473670137 title "Characterization of Dye-decolorizing Peroxidase (DyP) from Thermomonospora curvata Reveals Unique Catalytic Properties of A-type DyPs" @default.
- W1473670137 cites W1491373961 @default.
- W1473670137 cites W1500539090 @default.
- W1473670137 cites W1530595315 @default.
- W1473670137 cites W1531643941 @default.
- W1473670137 cites W1543033526 @default.
- W1473670137 cites W1569645121 @default.
- W1473670137 cites W1571455001 @default.
- W1473670137 cites W1799674254 @default.
- W1473670137 cites W1803620352 @default.
- W1473670137 cites W1907951436 @default.
- W1473670137 cites W1965114473 @default.
- W1473670137 cites W1967821193 @default.
- W1473670137 cites W1968081696 @default.
- W1473670137 cites W1973759673 @default.
- W1473670137 cites W1973839344 @default.
- W1473670137 cites W1975206994 @default.
- W1473670137 cites W1978950679 @default.
- W1473670137 cites W1986619637 @default.
- W1473670137 cites W1990205765 @default.
- W1473670137 cites W1994849220 @default.
- W1473670137 cites W1995002017 @default.
- W1473670137 cites W1996181006 @default.
- W1473670137 cites W2000508647 @default.
- W1473670137 cites W2002065095 @default.
- W1473670137 cites W2002159869 @default.
- W1473670137 cites W2008610282 @default.
- W1473670137 cites W2014944909 @default.
- W1473670137 cites W2018661561 @default.
- W1473670137 cites W2022470565 @default.
- W1473670137 cites W2026182346 @default.
- W1473670137 cites W2028894012 @default.
- W1473670137 cites W2029441786 @default.
- W1473670137 cites W2036087350 @default.
- W1473670137 cites W2038529067 @default.
- W1473670137 cites W2039875881 @default.
- W1473670137 cites W2040625048 @default.
- W1473670137 cites W2044423696 @default.
- W1473670137 cites W2048459982 @default.
- W1473670137 cites W2050384759 @default.
- W1473670137 cites W2062303947 @default.
- W1473670137 cites W2074067692 @default.
- W1473670137 cites W2074336931 @default.
- W1473670137 cites W2079906167 @default.
- W1473670137 cites W2080037118 @default.
- W1473670137 cites W2081915841 @default.
- W1473670137 cites W2083071890 @default.
- W1473670137 cites W2083297860 @default.
- W1473670137 cites W2090933843 @default.
- W1473670137 cites W2095100183 @default.
- W1473670137 cites W2096330754 @default.
- W1473670137 cites W2106603721 @default.
- W1473670137 cites W2107277439 @default.
- W1473670137 cites W2119292314 @default.
- W1473670137 cites W2123699036 @default.
- W1473670137 cites W2128536241 @default.
- W1473670137 cites W2138728991 @default.
- W1473670137 cites W2140538197 @default.
- W1473670137 cites W2149293626 @default.
- W1473670137 cites W2151888107 @default.
- W1473670137 cites W2165898505 @default.
- W1473670137 cites W2167684533 @default.
- W1473670137 cites W2320996430 @default.
- W1473670137 cites W2321127140 @default.
- W1473670137 cites W2321627128 @default.
- W1473670137 cites W4232534952 @default.
- W1473670137 cites W4253195366 @default.
- W1473670137 doi "https://doi.org/10.1074/jbc.m115.658807" @default.
- W1473670137 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4645587" @default.
- W1473670137 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26205819" @default.
- W1473670137 hasPublicationYear "2015" @default.
- W1473670137 type Work @default.
- W1473670137 sameAs 1473670137 @default.
- W1473670137 citedByCount "92" @default.
- W1473670137 countsByYear W14736701372016 @default.
- W1473670137 countsByYear W14736701372017 @default.
- W1473670137 countsByYear W14736701372018 @default.
- W1473670137 countsByYear W14736701372019 @default.
- W1473670137 countsByYear W14736701372020 @default.
- W1473670137 countsByYear W14736701372021 @default.
- W1473670137 countsByYear W14736701372022 @default.
- W1473670137 countsByYear W14736701372023 @default.
- W1473670137 crossrefType "journal-article" @default.
- W1473670137 hasAuthorship W1473670137A5002389016 @default.
- W1473670137 hasAuthorship W1473670137A5042287156 @default.
- W1473670137 hasAuthorship W1473670137A5048246567 @default.