Matches in SemOpenAlex for { <https://semopenalex.org/work/W147496640> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W147496640 endingPage "9" @default.
- W147496640 startingPage "54" @default.
- W147496640 abstract "C-erbB-2 is a chief proto-oncogene of endometrial cancer, which plays an important role in the biological behavior of endometrial cancer. Its overespression is an important factor of poor progrosis. The objective of this study was to investigate the treatment effects of transfecting c-erbB-2 antisense oligonucleotide (ASODN) on the uterine endometrial cancer Ishikawa cell line.The c-erbB-2 expression on Ishikawa cell membranes was determined by immunohistochemistry and then the aim was to transfect ASODN into Ishikawa cells and assay the cellular growth inhibition by MTT, to observe the cellular ultrastructure changes under transmission electron microscope (TEM), and to assay the cellular apoptotic rate, c-erbB-2 mRNA and protein expression by flow cytometry, RT-PCR and Western blot, respectively.C-erbB-2 protein was positively expressed on Ishikawa cell membranes. MTT showed that when the concentration of transfecting ASODNs was 0.3 uM and 0.6 uM, cell growth inhibition rates were 55.43% and 76.12%, respectively. After transfecting 0.3 uM ASODN, the Ishikawa cell ultrastructure was obviously damaged, the cellular apoptotic rate was 72.21%, and the c-erbB-2 mRNA and protein expression were 45.71% and 34.52%, respectively, compared with those of the normal control cells.Transfecting c-erbB-2 ASODN can obviously suppress its mRNA and protein expression in Ishikawa cells, cause cellular apoptosis and inhibit cell growth. It may have an important role in the gene therapy of endometrial cancer." @default.
- W147496640 created "2016-06-24" @default.
- W147496640 creator A5022298405 @default.
- W147496640 creator A5027444774 @default.
- W147496640 creator A5029475326 @default.
- W147496640 creator A5038694989 @default.
- W147496640 creator A5048568839 @default.
- W147496640 date "2009-01-01" @default.
- W147496640 modified "2023-09-27" @default.
- W147496640 title "Inhibitory effects of c-erbB-2 antisense oligonucleotide transfection on uterine endometrial cancer Ishikawa cell lines." @default.
- W147496640 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19317258" @default.
- W147496640 hasPublicationYear "2009" @default.
- W147496640 type Work @default.
- W147496640 sameAs 147496640 @default.
- W147496640 citedByCount "3" @default.
- W147496640 countsByYear W1474966402012 @default.
- W147496640 countsByYear W1474966402013 @default.
- W147496640 countsByYear W1474966402022 @default.
- W147496640 crossrefType "journal-article" @default.
- W147496640 hasAuthorship W147496640A5022298405 @default.
- W147496640 hasAuthorship W147496640A5027444774 @default.
- W147496640 hasAuthorship W147496640A5029475326 @default.
- W147496640 hasAuthorship W147496640A5038694989 @default.
- W147496640 hasAuthorship W147496640A5048568839 @default.
- W147496640 hasConcept C121608353 @default.
- W147496640 hasConcept C126322002 @default.
- W147496640 hasConcept C1491633281 @default.
- W147496640 hasConcept C153911025 @default.
- W147496640 hasConcept C190283241 @default.
- W147496640 hasConcept C2777088508 @default.
- W147496640 hasConcept C2780783641 @default.
- W147496640 hasConcept C2781018059 @default.
- W147496640 hasConcept C29537977 @default.
- W147496640 hasConcept C502942594 @default.
- W147496640 hasConcept C54009773 @default.
- W147496640 hasConcept C54355233 @default.
- W147496640 hasConcept C553184892 @default.
- W147496640 hasConcept C55493867 @default.
- W147496640 hasConcept C62112901 @default.
- W147496640 hasConcept C71924100 @default.
- W147496640 hasConcept C81885089 @default.
- W147496640 hasConcept C86803240 @default.
- W147496640 hasConceptScore W147496640C121608353 @default.
- W147496640 hasConceptScore W147496640C126322002 @default.
- W147496640 hasConceptScore W147496640C1491633281 @default.
- W147496640 hasConceptScore W147496640C153911025 @default.
- W147496640 hasConceptScore W147496640C190283241 @default.
- W147496640 hasConceptScore W147496640C2777088508 @default.
- W147496640 hasConceptScore W147496640C2780783641 @default.
- W147496640 hasConceptScore W147496640C2781018059 @default.
- W147496640 hasConceptScore W147496640C29537977 @default.
- W147496640 hasConceptScore W147496640C502942594 @default.
- W147496640 hasConceptScore W147496640C54009773 @default.
- W147496640 hasConceptScore W147496640C54355233 @default.
- W147496640 hasConceptScore W147496640C553184892 @default.
- W147496640 hasConceptScore W147496640C55493867 @default.
- W147496640 hasConceptScore W147496640C62112901 @default.
- W147496640 hasConceptScore W147496640C71924100 @default.
- W147496640 hasConceptScore W147496640C81885089 @default.
- W147496640 hasConceptScore W147496640C86803240 @default.
- W147496640 hasIssue "1" @default.
- W147496640 hasLocation W1474966401 @default.
- W147496640 hasOpenAccess W147496640 @default.
- W147496640 hasPrimaryLocation W1474966401 @default.
- W147496640 hasRelatedWork W1768871930 @default.
- W147496640 hasRelatedWork W1972317737 @default.
- W147496640 hasRelatedWork W2036381463 @default.
- W147496640 hasRelatedWork W2149203329 @default.
- W147496640 hasRelatedWork W2323137446 @default.
- W147496640 hasRelatedWork W2362313441 @default.
- W147496640 hasRelatedWork W2364623278 @default.
- W147496640 hasRelatedWork W2373824398 @default.
- W147496640 hasRelatedWork W2387420200 @default.
- W147496640 hasRelatedWork W2411509593 @default.
- W147496640 hasRelatedWork W2411791818 @default.
- W147496640 hasRelatedWork W2411988918 @default.
- W147496640 hasRelatedWork W2418304623 @default.
- W147496640 hasRelatedWork W2419248463 @default.
- W147496640 hasRelatedWork W3030156323 @default.
- W147496640 hasRelatedWork W3030333658 @default.
- W147496640 hasRelatedWork W3031916006 @default.
- W147496640 hasRelatedWork W3140140071 @default.
- W147496640 hasRelatedWork W3147843563 @default.
- W147496640 hasRelatedWork W94744195 @default.
- W147496640 hasVolume "30" @default.
- W147496640 isParatext "false" @default.
- W147496640 isRetracted "false" @default.
- W147496640 magId "147496640" @default.
- W147496640 workType "article" @default.