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- W1475193419 abstract "The term “protein engineering” refers to the systematic replacement of amino acid residues to alter the properties of a protein. There are currently two approaches for determining quantitatively the importance of charge–charge interactions in proteins: experimental and theoretical. This chapter is concerned with an experimental, rational method for the determination and manipulation of electrostatic effects in enzymes. It also presents a model system to study the magnitude of electrostatic interactions whose characteristics are the following: (1) a known three-dimensional structure with sufficient resolution for the analysis of the effect of mutations on the water structure; (2) an available expression system for the protein; (3) a well-characterized mechanism; (4) a relatively simple, accurate, and precise assay; (5) the availability of a variety of different substrates; and the dependence of a number of properties on charge–charge interactions." @default.
- W1475193419 created "2016-06-24" @default.
- W1475193419 creator A5002426532 @default.
- W1475193419 creator A5065989067 @default.
- W1475193419 date "1991-01-01" @default.
- W1475193419 modified "2023-09-25" @default.
- W1475193419 title "[27] Modification of enzyme catalysis by engineering surface charge" @default.
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- W1475193419 doi "https://doi.org/10.1016/0076-6879(91)02029-9" @default.
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