Matches in SemOpenAlex for { <https://semopenalex.org/work/W147691984> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W147691984 abstract "Oligonucleotides containing black hole quenchers are widely used as the probes for quantitative and RT-PCR applications. For reproducible and stable PCR results it is essential to use high grade probes, which makes oligonucleotide purification and QC steps important for success. Typical methods, such as HPLC and capillary electrophoresis yield information on overall purity of the probe, while mass spectrometry provides more chemical information hardly accessible by other analytical techniques, such as nature of synthetic failure products, degree of deprotection, presence of deterioration products due to apurinization etc. While MALDI-TOF mass spectrometry is a method of choice for QC of synthetic oligonucleotides it?s application toward modified oligonucleotides containing dark quenchers is limited because of complication of spectra due to photo-dissociation of quencher group during desorption-ionization step. These in-plume photo-decomposition reactions lead to appearance of spectral artifacts interferencing with the actual signals and lead to more propensity for peak broadening at elevated laser powers. Such effects are observable for both nitrogen gas (337nm) and solid Nd-YAG (355nm) lasers typically used in MALDI mass spectrometers. In this work we discussed mechanism of photo-degradation and investigated various ways to minimize it. Short oligonucleotides containing BHQ-1, BHQ-2, and BHQ-3 labels were synthesized for testing purposes. The photo-fragmentation degree was evaluated in presence of various MALDI matrices. Different approaches to quench the exited states of quencher groups during ionization were investigated. That includes collisional cooling by using neutral additives (such as sugars) in order to facilitate energy dissipation from BHQ-groups and application of various organic additives bearing red-ox functionality, which could quench in-plume exited triplet states of azido groups. The novel matrix composition has been developed, which inhibits the photo-fragmentation of BHQ-groups during desorption-ionization step, thus eliminating spectrum artifacts and allows for successful analysis of BHQ-labeled oligonucleotides by MALDI MS." @default.
- W147691984 created "2016-06-24" @default.
- W147691984 creator A5030141329 @default.
- W147691984 creator A5071336235 @default.
- W147691984 date "2013-05-01" @default.
- W147691984 modified "2023-09-22" @default.
- W147691984 title "MALDI Analysis of Modified Oligonucleotides Containing Dark Quenchers" @default.
- W147691984 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3635290" @default.
- W147691984 hasPublicationYear "2013" @default.
- W147691984 type Work @default.
- W147691984 sameAs 147691984 @default.
- W147691984 citedByCount "0" @default.
- W147691984 crossrefType "journal-article" @default.
- W147691984 hasAuthorship W147691984A5030141329 @default.
- W147691984 hasAuthorship W147691984A5071336235 @default.
- W147691984 hasConcept C102931765 @default.
- W147691984 hasConcept C111919701 @default.
- W147691984 hasConcept C113196181 @default.
- W147691984 hasConcept C129312508 @default.
- W147691984 hasConcept C145148216 @default.
- W147691984 hasConcept C150394285 @default.
- W147691984 hasConcept C162356407 @default.
- W147691984 hasConcept C162711632 @default.
- W147691984 hasConcept C178790620 @default.
- W147691984 hasConcept C185592680 @default.
- W147691984 hasConcept C191015642 @default.
- W147691984 hasConcept C198291218 @default.
- W147691984 hasConcept C40325409 @default.
- W147691984 hasConcept C41008148 @default.
- W147691984 hasConcept C43617362 @default.
- W147691984 hasConcept C552990157 @default.
- W147691984 hasConcept C55493867 @default.
- W147691984 hasConceptScore W147691984C102931765 @default.
- W147691984 hasConceptScore W147691984C111919701 @default.
- W147691984 hasConceptScore W147691984C113196181 @default.
- W147691984 hasConceptScore W147691984C129312508 @default.
- W147691984 hasConceptScore W147691984C145148216 @default.
- W147691984 hasConceptScore W147691984C150394285 @default.
- W147691984 hasConceptScore W147691984C162356407 @default.
- W147691984 hasConceptScore W147691984C162711632 @default.
- W147691984 hasConceptScore W147691984C178790620 @default.
- W147691984 hasConceptScore W147691984C185592680 @default.
- W147691984 hasConceptScore W147691984C191015642 @default.
- W147691984 hasConceptScore W147691984C198291218 @default.
- W147691984 hasConceptScore W147691984C40325409 @default.
- W147691984 hasConceptScore W147691984C41008148 @default.
- W147691984 hasConceptScore W147691984C43617362 @default.
- W147691984 hasConceptScore W147691984C552990157 @default.
- W147691984 hasConceptScore W147691984C55493867 @default.
- W147691984 hasLocation W1476919841 @default.
- W147691984 hasOpenAccess W147691984 @default.
- W147691984 hasPrimaryLocation W1476919841 @default.
- W147691984 hasRelatedWork W1470624736 @default.
- W147691984 hasRelatedWork W1490720951 @default.
- W147691984 hasRelatedWork W1966070434 @default.
- W147691984 hasRelatedWork W1980885203 @default.
- W147691984 hasRelatedWork W1989488854 @default.
- W147691984 hasRelatedWork W1994075320 @default.
- W147691984 hasRelatedWork W1996724572 @default.
- W147691984 hasRelatedWork W2010341439 @default.
- W147691984 hasRelatedWork W2023171189 @default.
- W147691984 hasRelatedWork W2052734047 @default.
- W147691984 hasRelatedWork W2058140970 @default.
- W147691984 hasRelatedWork W2063546722 @default.
- W147691984 hasRelatedWork W2090277609 @default.
- W147691984 hasRelatedWork W2166997430 @default.
- W147691984 hasRelatedWork W2169201093 @default.
- W147691984 hasRelatedWork W2226038589 @default.
- W147691984 hasRelatedWork W2559442466 @default.
- W147691984 hasRelatedWork W2784973953 @default.
- W147691984 hasRelatedWork W3206410819 @default.
- W147691984 hasRelatedWork W2832180777 @default.
- W147691984 hasVolume "24" @default.
- W147691984 isParatext "false" @default.
- W147691984 isRetracted "false" @default.
- W147691984 magId "147691984" @default.
- W147691984 workType "article" @default.