Matches in SemOpenAlex for { <https://semopenalex.org/work/W147745229> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W147745229 endingPage "S217" @default.
- W147745229 startingPage "S217" @default.
- W147745229 abstract "Abstract Human CR2, a receptor for complement C3d, EBV-gp350, CD23 and IFN-ƒÑ is a B lymphocyte membrane glycoprotein that plays a central role in immunity and autoimmunity. Dysfunction or absence of CR2 promotes development of anti-DNA antibodies in models of systemic lupus erythematosus. In this study, we show that CpG ODN and TLR9 bind to CR2 using ELISA and surface plasmon resonance (SPR). Using SPR, in which carboxy-terminal biotin-tagged CR2 SCR1-4 on a streptavidin chip and CpG ODN and TLR9 as solution phase analyte were studied, the calculated equilibrium dissociation constant (kd/ka) for this binding, reveal 3.1¡Ñ10-2/5.4¡Ñ10-5 s-1 and /1.1¡Ñ10-2/2.4¡Ñ106 s-1 M-1 with a maximal KD of 571 nM and 42 nM respectively. Monoclonal antibodies developed against the first 2 short consensus repeats (SCR) blocked full-length CR2 binding to CpG ODN and TLR9 by ELISA, identically to their effect on C3d binding, indicating the first two SCR involved in this binding. Further more, treatment with chloroquine, a known inhibitor of TLR9 signaling blocks the CR2-TLR9 interaction by ELISA. We are currently determining whether, by using activation of Namalwa B cells with CpG ODN, blockade of CR2 inhibits the CpG-induced NF-kB activation and induction of IL-6 release. These data suggest that CR2 may functionally interact with TLR9 on B cells." @default.
- W147745229 created "2016-06-24" @default.
- W147745229 creator A5001787457 @default.
- W147745229 creator A5002184526 @default.
- W147745229 creator A5009539262 @default.
- W147745229 date "2007-04-01" @default.
- W147745229 modified "2023-09-27" @default.
- W147745229 title "CpG ODN-induced NF-kB activation / IL-6 secretion through CD21-TLR9 dependent B cell signaling? (128.34)" @default.
- W147745229 doi "https://doi.org/10.4049/jimmunol.178.supp.128.34" @default.
- W147745229 hasPublicationYear "2007" @default.
- W147745229 type Work @default.
- W147745229 sameAs 147745229 @default.
- W147745229 citedByCount "0" @default.
- W147745229 crossrefType "journal-article" @default.
- W147745229 hasAuthorship W147745229A5001787457 @default.
- W147745229 hasAuthorship W147745229A5002184526 @default.
- W147745229 hasAuthorship W147745229A5009539262 @default.
- W147745229 hasConcept C104317684 @default.
- W147745229 hasConcept C140173407 @default.
- W147745229 hasConcept C150194340 @default.
- W147745229 hasConcept C153911025 @default.
- W147745229 hasConcept C185592680 @default.
- W147745229 hasConcept C190727270 @default.
- W147745229 hasConcept C22801619 @default.
- W147745229 hasConcept C2779138994 @default.
- W147745229 hasConcept C2908999202 @default.
- W147745229 hasConcept C55493867 @default.
- W147745229 hasConcept C86803240 @default.
- W147745229 hasConceptScore W147745229C104317684 @default.
- W147745229 hasConceptScore W147745229C140173407 @default.
- W147745229 hasConceptScore W147745229C150194340 @default.
- W147745229 hasConceptScore W147745229C153911025 @default.
- W147745229 hasConceptScore W147745229C185592680 @default.
- W147745229 hasConceptScore W147745229C190727270 @default.
- W147745229 hasConceptScore W147745229C22801619 @default.
- W147745229 hasConceptScore W147745229C2779138994 @default.
- W147745229 hasConceptScore W147745229C2908999202 @default.
- W147745229 hasConceptScore W147745229C55493867 @default.
- W147745229 hasConceptScore W147745229C86803240 @default.
- W147745229 hasIssue "1_Supplement" @default.
- W147745229 hasLocation W1477452291 @default.
- W147745229 hasOpenAccess W147745229 @default.
- W147745229 hasPrimaryLocation W1477452291 @default.
- W147745229 hasRelatedWork W1710348905 @default.
- W147745229 hasRelatedWork W2042437855 @default.
- W147745229 hasRelatedWork W2065193095 @default.
- W147745229 hasRelatedWork W2092470514 @default.
- W147745229 hasRelatedWork W2096956789 @default.
- W147745229 hasRelatedWork W2148845369 @default.
- W147745229 hasRelatedWork W2273855720 @default.
- W147745229 hasRelatedWork W2314264549 @default.
- W147745229 hasRelatedWork W2900166117 @default.
- W147745229 hasRelatedWork W3097231610 @default.
- W147745229 hasVolume "178" @default.
- W147745229 isParatext "false" @default.
- W147745229 isRetracted "false" @default.
- W147745229 magId "147745229" @default.
- W147745229 workType "article" @default.