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- W147830655 abstract "The mite Sarcoptes scabiei (Acari), causes sarcoptic mange or scabies that globally affects animals and humans. Although scabies and mange are recognised as important diseases in human and veterinary medicine the amount of molecular studies of S. scabiei have been limited, which has been attributed to a shortage of parasitic material. This thesis is based on four studies that make use of molecular techniques with the aims to overcome the problems associated with a scarcity of mites, gain insights into the genetic background of the mite and to identify proteins important for host-parasite interactions. A better knowledge of parasite proteins that interact with the host is pivotal for understanding the mite’s pathogenicity. In order to accelerate gene discovery in S. scabiei an expressed sequence tag (EST) analysis of 1,020 ESTs was performed. Around half of the ESTs could be assigned with a putative gene identity. In the data set several proteases and allergens were identified, all of which possibly are involved in host-parasite interactions. The EST analysis identified a transcript corresponding to the enzyme gluthathione-S-transerase (GST). GST has an active role in detoxification, and has been a target molecule for vaccine development and drug resistance in other parasitic diseases. A phylogentic study showed that it was a delta-class GST, which previously never had been described in the order Acari. Several recombinant versions of the delta-GST were expressed and all were enzymatically active. Paramyosin, major Sarcoptes antigen 1 (MSA1) and atypical Sarcoptes antigen 1 (ASA1), were identified by immunoscreening, and later expressed as recombinant proteins for further characterisation. Paramyosin is an invertebrate muscle protein, associated with protective immunity in helminth infections. Sera from both dogs and pigs infected with S. scabiei reacted with recombinant paramyosin. ASA1 contained a MADF domain, which is not normally associated with antigens. In a Western blot analysis, 24% of the S. scabiei positive dogs were positive to ASA1. In contrast 82% of the dogs had antibodies towards MSA1. Immunohistochemistry (IHC) localisation of ASA1 and MSA1 supports the observation that these proteins are exposed to the host. In contrast, IHC showed that delta-GST is more confined to the mites." @default.
- W147830655 created "2016-06-24" @default.
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- W147830655 date "2005-04-01" @default.
- W147830655 modified "2023-09-23" @default.
- W147830655 title "Molecular analysis of Sarcoptes scabiei" @default.
- W147830655 hasPublicationYear "2005" @default.
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