FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1479683678> ?p ?o ?g. }

• W1479683678 endingPage "3096" @default.
• W1479683678 startingPage "3092" @default.
• W1479683678 abstract "Half-lives of Bacillus alpha-amylases at 90 degrees C and pH 6.5 greatly increase in the series from Bacillus amyloliquefaciens to Bacillus stearothermophilus to Bacillus licheniformis, e.g. the difference in thermostability between the first and the third enzymes exceeds 2 orders of magnitude. This stabilization is achieved by lowering the rate constant of monomolecular conformational scrambling, which is the cause of irreversible thermoinactivation of B. amyloliquefaciens and B. stearothermophilus alpha-amylases, so that for B. licheniformis alpha-amylase, another process, deamidation of Asn/Gln residues, emerges as the cause of inactivation. The extra thermostability of the thermophilic enzyme was found to be mainly due to additional salt bridges involving a few specific lysine residues (Lys-385 and Lys-88 and/or Lys-253). These stabilizing electrostatic interactions reduce the extent of unfolding of the enzyme molecule at high temperatures, consequently making it less prone to forming incorrect (scrambled) structures and thus decreasing the overall rate of irreversible thermoinactivation. The implications of these findings for protein engineering are discussed." @default.
• W1479683678 created "2016-06-24" @default.
• W1479683678 creator A5010204325 @default.
• W1479683678 creator A5023002381 @default.
• W1479683678 date "1988-03-01" @default.
• W1479683678 modified "2023-10-16" @default.
• W1479683678 title "Why is one Bacillus alpha-amylase more resistant against irreversible thermoinactivation than another?" @default.
• W1479683678 cites W1485865656 @default.
• W1479683678 cites W17993861 @default.
• W1479683678 cites W1851792052 @default.
• W1479683678 cites W1874832326 @default.
• W1479683678 cites W1966302520 @default.
• W1479683678 cites W1969136384 @default.
• W1479683678 cites W1975284238 @default.
• W1479683678 cites W1997619498 @default.
• W1479683678 cites W2004773817 @default.
• W1479683678 cites W2014316303 @default.
• W1479683678 cites W2026530009 @default.
• W1479683678 cites W2027027784 @default.
• W1479683678 cites W2028928741 @default.
• W1479683678 cites W2029060458 @default.
• W1479683678 cites W2030456541 @default.
• W1479683678 cites W2035283686 @default.
• W1479683678 cites W2040351161 @default.
• W1479683678 cites W2056954807 @default.
• W1479683678 cites W205739566 @default.
• W1479683678 cites W2065051737 @default.
• W1479683678 cites W2075354188 @default.
• W1479683678 cites W2078720851 @default.
• W1479683678 cites W2091381149 @default.
• W1479683678 cites W2098807743 @default.
• W1479683678 cites W2109416507 @default.
• W1479683678 cites W2141420244 @default.
• W1479683678 cites W2150003941 @default.
• W1479683678 cites W2334561852 @default.
• W1479683678 cites W2916050334 @default.
• W1479683678 cites W4210948091 @default.
• W1479683678 cites W4211239749 @default.
• W1479683678 cites W4293247451 @default.
• W1479683678 cites W99440142 @default.
• W1479683678 doi "https://doi.org/10.1016/s0021-9258(18)69039-8" @default.
• W1479683678 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3125174" @default.
• W1479683678 hasPublicationYear "1988" @default.
• W1479683678 type Work @default.
• W1479683678 sameAs 1479683678 @default.
• W1479683678 citedByCount "158" @default.
• W1479683678 countsByYear W14796836782012 @default.
• W1479683678 countsByYear W14796836782013 @default.
• W1479683678 countsByYear W14796836782014 @default.
• W1479683678 countsByYear W14796836782015 @default.
• W1479683678 countsByYear W14796836782016 @default.
• W1479683678 countsByYear W14796836782017 @default.
• W1479683678 countsByYear W14796836782018 @default.
• W1479683678 countsByYear W14796836782019 @default.
• W1479683678 countsByYear W14796836782020 @default.
• W1479683678 countsByYear W14796836782021 @default.
• W1479683678 crossrefType "journal-article" @default.
• W1479683678 hasAuthorship W1479683678A5010204325 @default.
• W1479683678 hasAuthorship W1479683678A5023002381 @default.
• W1479683678 hasBestOaLocation W14796836781 @default.
• W1479683678 hasConcept C105795698 @default.
• W1479683678 hasConcept C170835558 @default.
• W1479683678 hasConcept C171606756 @default.
• W1479683678 hasConcept C173959671 @default.
• W1479683678 hasConcept C181199279 @default.
• W1479683678 hasConcept C185592680 @default.
• W1479683678 hasConcept C33923547 @default.
• W1479683678 hasConcept C49453240 @default.
• W1479683678 hasConcept C55493867 @default.
• W1479683678 hasConcept C64943373 @default.
• W1479683678 hasConcept C86803240 @default.
• W1479683678 hasConcept C89423630 @default.
• W1479683678 hasConceptScore W1479683678C105795698 @default.
• W1479683678 hasConceptScore W1479683678C170835558 @default.
• W1479683678 hasConceptScore W1479683678C171606756 @default.
• W1479683678 hasConceptScore W1479683678C173959671 @default.
• W1479683678 hasConceptScore W1479683678C181199279 @default.
• W1479683678 hasConceptScore W1479683678C185592680 @default.
• W1479683678 hasConceptScore W1479683678C33923547 @default.
• W1479683678 hasConceptScore W1479683678C49453240 @default.
• W1479683678 hasConceptScore W1479683678C55493867 @default.
• W1479683678 hasConceptScore W1479683678C64943373 @default.
• W1479683678 hasConceptScore W1479683678C86803240 @default.
• W1479683678 hasConceptScore W1479683678C89423630 @default.
• W1479683678 hasIssue "7" @default.
• W1479683678 hasLocation W14796836781 @default.
• W1479683678 hasOpenAccess W1479683678 @default.
• W1479683678 hasPrimaryLocation W14796836781 @default.
• W1479683678 hasRelatedWork W14210377 @default.
• W1479683678 hasRelatedWork W2016706335 @default.
• W1479683678 hasRelatedWork W2020936318 @default.
• W1479683678 hasRelatedWork W2051240707 @default.
• W1479683678 hasRelatedWork W2467372068 @default.
• W1479683678 hasRelatedWork W2787792203 @default.
• W1479683678 hasRelatedWork W3175406851 @default.
• W1479683678 hasRelatedWork W4237269082 @default.
• W1479683678 hasRelatedWork W4250051680 @default.
• W1479683678 hasRelatedWork W4299430233 @default.

• next