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- W1480277779 abstract "Micropipette-tip solid phase extraction systems are common in proteomic analyses for desalting and concentrating samples for mass spectrometry, removing interferences, and increasing sensitivity. These systems are inexpensive, disposable, and highly efficient. Here we show micropipette-tip solid phase extraction is a direct sample preparation method for {sup 14}C-accelerator mass spectrometry (AMS), removing salts or reagent from labeled macromolecules. We compared loading, recovery and desalting efficiency in commercially available SPE micro-tips using {sup 14}C-labeled peptides and proteins, AMS, and alpha spectrometry ion energy loss quantitation. The polypropylene in the tips was nearly {sup 14}C-free and simultaneously provided low-background carrier for AMS. The silica material did not interfere with the analysis. Alpha spectrometry provided an absolute measurement of desalting efficiency." @default.
- W1480277779 created "2016-06-24" @default.
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- W1480277779 date "2004-08-02" @default.
- W1480277779 modified "2023-09-27" @default.
- W1480277779 title "Quantitation of Binding, Recovery and Desalting Efficiency in Solid Phase Extraction Micropipette Tips" @default.
- W1480277779 hasPublicationYear "2004" @default.
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