FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1480402597> ?p ?o ?g. }

• W1480402597 endingPage "5138" @default.
• W1480402597 startingPage "5126" @default.
• W1480402597 abstract "The pattern of induced protein species of the prototype strain of the parvovirus minute virus of mice was determined in permissive A9 mouse fibroblast cells by high-resolution two-dimensional gel electrophoresis. Identities of the viral proteins in the gels were assigned by probing two-dimensional blots with antisera raised against either purified capsids (recognizing VP-1 and VP-2) or specific coding regions of the nonstructural proteins (NS-1 and NS-2) expressed as beta-galactosidase fusion products in bacteria. All viral proteins showed posttranslational modifications, phosphate being a common substituent. The NS-1 protein migrated as a basic polypeptide in the pI range of 7.4 to 7.8 with multiple stages of modification and as a likely minor but hyperphosphorylated component in the neutral region of the gel. The NS-2 isoforms were resolved at a pI value close to 5.5 as three groups of unevenly phosphorylated polypeptides, each composed of at least two protein species. Both VP-1 and VP-2 structural polypeptides were induced as heterogeneous phosphoproteins. The major VP-2 protein could be resolved in the form of a consistent pattern of three abundant (a to c), two intermediate (d and e), and one meager (f) neutral isoelectric focusing species or subtypes. This posttranslational modification precedes and is uncoupled from viral assembly, and all of the VP-2 subtypes are packaged into empty capsids at the induced stoichiometry. However, intracellular full virions harbored additional phosphorylated subtypes (g to l) and a subtle rearrangement in the whole VP-2 composition, while mature virions purified from lysed cultures lacked these subtypes, coordinately with the emergence of six neutral VP-3 subtypes. Thus, the virion coat undergoes a chemical transition entailed by genome encapsidation, in which phosphates seem to play a major role, triggering the preferential proteolytic cleavage of the more acidic VP-2 subtypes to VP-3. Parvoviruses, with small coding capacity, may regulate some morphogenetic steps, such as assembly, genome encapsidation, and maturation, by posttranslational modifications of their structural proteins." @default.
• W1480402597 created "2016-06-24" @default.
• W1480402597 creator A5032172495 @default.
• W1480402597 creator A5035197458 @default.
• W1480402597 creator A5091282555 @default.
• W1480402597 date "1993-09-01" @default.
• W1480402597 modified "2023-09-26" @default.
• W1480402597 title "Protein species of the parvovirus minute virus of mice strain MVMp: involvement of phosphorylated VP-2 subtypes in viral morphogenesis" @default.
• W1480402597 cites W1030199751 @default.
• W1480402597 cites W1415981329 @default.
• W1480402597 cites W1484473517 @default.
• W1480402597 cites W1492841822 @default.
• W1480402597 cites W1506805850 @default.
• W1480402597 cites W1529305757 @default.
• W1480402597 cites W1535892295 @default.
• W1480402597 cites W1552232782 @default.
• W1480402597 cites W1553910668 @default.
• W1480402597 cites W1560427600 @default.
• W1480402597 cites W1570016597 @default.
• W1480402597 cites W1577471567 @default.
• W1480402597 cites W1588017982 @default.
• W1480402597 cites W1605162382 @default.
• W1480402597 cites W1606423922 @default.
• W1480402597 cites W1608856256 @default.
• W1480402597 cites W1642761548 @default.
• W1480402597 cites W1657059619 @default.
• W1480402597 cites W1657659731 @default.
• W1480402597 cites W1674276687 @default.
• W1480402597 cites W1689794862 @default.
• W1480402597 cites W1795729066 @default.
• W1480402597 cites W1797747900 @default.
• W1480402597 cites W1814543726 @default.
• W1480402597 cites W1827825926 @default.
• W1480402597 cites W190650499 @default.
• W1480402597 cites W1919785074 @default.
• W1480402597 cites W1940048575 @default.
• W1480402597 cites W1940768144 @default.
• W1480402597 cites W1956123817 @default.
• W1480402597 cites W1957453673 @default.
• W1480402597 cites W1958499520 @default.
• W1480402597 cites W1971523756 @default.
• W1480402597 cites W1972168467 @default.
• W1480402597 cites W1972542571 @default.
• W1480402597 cites W1981157017 @default.
• W1480402597 cites W1986585414 @default.
• W1480402597 cites W1989711551 @default.
• W1480402597 cites W1994239463 @default.
• W1480402597 cites W1999739269 @default.
• W1480402597 cites W2001085828 @default.
• W1480402597 cites W2006388592 @default.
• W1480402597 cites W2006766410 @default.
• W1480402597 cites W2008688413 @default.
• W1480402597 cites W2018289835 @default.
• W1480402597 cites W2029675011 @default.
• W1480402597 cites W2030751641 @default.
• W1480402597 cites W2040497667 @default.
• W1480402597 cites W2049651959 @default.
• W1480402597 cites W2057347572 @default.
• W1480402597 cites W2060143053 @default.
• W1480402597 cites W2066748158 @default.
• W1480402597 cites W2071390861 @default.
• W1480402597 cites W2076300996 @default.
• W1480402597 cites W2077537125 @default.
• W1480402597 cites W2079290149 @default.
• W1480402597 cites W2082890587 @default.
• W1480402597 cites W2083453910 @default.
• W1480402597 cites W2085347710 @default.
• W1480402597 cites W2087393962 @default.
• W1480402597 cites W2092061547 @default.
• W1480402597 cites W2101108802 @default.
• W1480402597 cites W2117337387 @default.
• W1480402597 cites W2119952624 @default.
• W1480402597 cites W2128072986 @default.
• W1480402597 cites W2128753750 @default.
• W1480402597 cites W2128984306 @default.
• W1480402597 cites W2142008563 @default.
• W1480402597 cites W2142332293 @default.
• W1480402597 cites W2146942707 @default.
• W1480402597 cites W2151095762 @default.
• W1480402597 cites W2157966332 @default.
• W1480402597 cites W2165110955 @default.
• W1480402597 cites W2167776440 @default.
• W1480402597 cites W2170174778 @default.
• W1480402597 cites W2199302867 @default.
• W1480402597 cites W2411936514 @default.
• W1480402597 cites W36715850 @default.
• W1480402597 cites W2045436225 @default.
• W1480402597 doi "https://doi.org/10.1128/jvi.67.9.5126-5138.1993" @default.
• W1480402597 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/237910" @default.
• W1480402597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8394440" @default.
• W1480402597 hasPublicationYear "1993" @default.
• W1480402597 type Work @default.
• W1480402597 sameAs 1480402597 @default.
• W1480402597 citedByCount "23" @default.
• W1480402597 countsByYear W14804025972012 @default.
• W1480402597 countsByYear W14804025972016 @default.
• W1480402597 countsByYear W14804025972018 @default.
• W1480402597 countsByYear W14804025972019 @default.

• next