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- W1480683221 abstract "The elongation step of transcription is a dynamic process that provides many opportunities for regulation. Biochemical analysis of this reaction has allowed transcript elongation to be staged into several distinct mechanistic steps, each of which can be assayed. The assays themselves are simple and technically straightforward, but their design and interpretation require some understanding of the transcript-elongation process. Many eukaryotic genes are subject to some form of regulation during the elongation process. The regulation is influenced by trans-acting factors that can stimulate or inhibit the activity of the transcribing RNA polymerase. This chapter highlights the various methods and the types of assays that can be used to identify such factors in eukaryotic cells. The chapter illustrates the elongation assays of one such factor, TFIIS. The process of transcript elongation is discussed, with a particular emphasis on the definition of some of the commonly used terms and the steps where transcription regulation might occur." @default.
- W1480683221 created "2016-06-24" @default.
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- W1480683221 date "1996-01-01" @default.
- W1480683221 modified "2023-09-24" @default.
- W1480683221 title "Identifying regulators of transcript elongation in eukaryotes" @default.
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- W1480683221 doi "https://doi.org/10.1016/s0076-6879(96)74034-x" @default.
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