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- W1480777739 abstract "Xenopus egg extracts initiate replication at specific origin sites within mammalian G1-phase nuclei. Similarly, S-phase extracts from Saccharomyces cerevisiae initiate DNA replication within yeast nuclei at specific yeast origin sequences. Here we show that Xenopus egg extracts can initiate DNA replication within G1-phase yeast nuclei but do not recognize yeast origin sequences. When G1-phase yeast nuclei were introduced into Xenopus egg extract, semiconservative, aphidicolin-sensitive DNA synthesis was induced after a brief lag period and was restricted to a single round of replication. The specificity of initiation within the yeast 2 μm plasmid as well as in the vicinity of the chromosomal origin ARS1 was evaluated by neutral two-dimensional gel electrophoresis of replication intermediates. At both locations, replication was found to initiate outside of the ARS element. Manipulation of both cis- and trans-acting elements in the yeast genome before introduction of nuclei into Xenopus egg extract may provide a system with which to elucidate the requirements for vertebrate origin recognition. J. Cell. Biochem. 80:73–84, 2000. © 2000 Wiley-Liss, Inc." @default.
- W1480777739 created "2016-06-24" @default.
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- W1480777739 date "2000-01-01" @default.
- W1480777739 modified "2023-10-01" @default.
- W1480777739 title "Initiation of DNA replication inSaccharomyces cerevisiae G1-phase nuclei byXenopus egg extract" @default.
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- W1480777739 doi "https://doi.org/10.1002/1097-4644(20010101)80:1<73::aid-jcb70>3.0.co;2-7" @default.
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