Matches in SemOpenAlex for { <https://semopenalex.org/work/W1480930112> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1480930112 endingPage "4677" @default.
- W1480930112 startingPage "4671" @default.
- W1480930112 abstract "Steroid-induced difference spectra have been used to examine the combination of cholesterol with adrenal mitochondrial cytochrome P-450 which participates in cholesterol side chain cleavage (P-450scc) and the depletion of cholesterol from the cytochrome which results from turnover of the enzyme system. Type I difference spectra-induced by cholest-5-ene-3beta, 25-diol (25-hydroxycholesterol) and cholest-5-ene-3beta, 20 alpha, 22R-triol (20alpha, 22R dihydroxycholesterol) have been used to quantitate binding of cholesterol to two sites (I and II) on cytochrome P-450scc. The action of adrenocorticotropic hormone (ACTH) in vivo and the action of calcium or phosphate ions on isolated mitochondria stimulate the combination of cholesterol with site I but not site II. Cholesterol derived from lecithin-cholesterol micelles, however, binds to both sites. Malate-induced cholesterol depletion occurred at a comparable rate to the transfer of cholesterol from lecithin-cholesterol micelles. However, a residual proportion of cholesterol-cytochrome P-450scc complexes remained, even after 10 min of exposure to malate, and was of similar magnitude in mitochondria from both cycloheximide-treated and stressed rats. It is suggested that this reflects a less reactive form of cholesterol-cytochrome complex. Steroid-induced difference spectra indicate that sites I and II on cytochrome P-450scc are similarly depleted after metabolism of mitochondrial cholesterol in vitro and after inhibition of the action of ACTH in vivo. Anaerobiosis of adrenal cells after excision of the accumulation of cholesterol at cytochrome P-450cc. When anaerobiosis was prevented, cytochrome P-450scc in the freshly isolated mitochondria was apparently essentially free of complexed cholesterol, irrespective of the extent of ACTH action. For 30 min after suspension of the mitochondria in 0.25 M sucrose at 4 degrees, cholesterol combines with cytochrome P-450scc. The extent of this process was not affected by the presence of cycloheximide during ether stress treatment of the rats. It is concluded that there are at least two pools of mitochondrial cholesterol with access to cytochrome P-450scc but that ACTH stimulates only the pool which most readily interacts with the cytochrome." @default.
- W1480930112 created "2016-06-24" @default.
- W1480930112 creator A5003497148 @default.
- W1480930112 creator A5016348153 @default.
- W1480930112 date "1975-06-01" @default.
- W1480930112 modified "2023-10-06" @default.
- W1480930112 title "Cytochrome P-450 of adrenal mitochondria. In vitro and in vivo changes in spin states." @default.
- W1480930112 cites W1028728095 @default.
- W1480930112 cites W1513897103 @default.
- W1480930112 cites W1556225860 @default.
- W1480930112 cites W1578839852 @default.
- W1480930112 cites W1596856156 @default.
- W1480930112 cites W179357761 @default.
- W1480930112 cites W1969730155 @default.
- W1480930112 cites W1970538646 @default.
- W1480930112 cites W1972321347 @default.
- W1480930112 cites W1976472536 @default.
- W1480930112 cites W1976749670 @default.
- W1480930112 cites W1977650403 @default.
- W1480930112 cites W1983135975 @default.
- W1480930112 cites W1983778724 @default.
- W1480930112 cites W1990567610 @default.
- W1480930112 cites W2019951606 @default.
- W1480930112 cites W2023523875 @default.
- W1480930112 cites W2046728975 @default.
- W1480930112 cites W2059229403 @default.
- W1480930112 cites W2059836007 @default.
- W1480930112 cites W2064764364 @default.
- W1480930112 cites W2080355919 @default.
- W1480930112 cites W2083702892 @default.
- W1480930112 cites W2138142730 @default.
- W1480930112 cites W2148865997 @default.
- W1480930112 cites W27405370 @default.
- W1480930112 doi "https://doi.org/10.1016/s0021-9258(19)41353-7" @default.
- W1480930112 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/167001" @default.
- W1480930112 hasPublicationYear "1975" @default.
- W1480930112 type Work @default.
- W1480930112 sameAs 1480930112 @default.
- W1480930112 citedByCount "47" @default.
- W1480930112 countsByYear W14809301122019 @default.
- W1480930112 countsByYear W14809301122021 @default.
- W1480930112 crossrefType "journal-article" @default.
- W1480930112 hasAuthorship W1480930112A5003497148 @default.
- W1480930112 hasAuthorship W1480930112A5016348153 @default.
- W1480930112 hasBestOaLocation W14809301121 @default.
- W1480930112 hasConcept C181199279 @default.
- W1480930112 hasConcept C185592680 @default.
- W1480930112 hasConcept C202751555 @default.
- W1480930112 hasConcept C207001950 @default.
- W1480930112 hasConcept C2780768313 @default.
- W1480930112 hasConcept C28859421 @default.
- W1480930112 hasConcept C54355233 @default.
- W1480930112 hasConcept C55493867 @default.
- W1480930112 hasConcept C86803240 @default.
- W1480930112 hasConcept C95444343 @default.
- W1480930112 hasConceptScore W1480930112C181199279 @default.
- W1480930112 hasConceptScore W1480930112C185592680 @default.
- W1480930112 hasConceptScore W1480930112C202751555 @default.
- W1480930112 hasConceptScore W1480930112C207001950 @default.
- W1480930112 hasConceptScore W1480930112C2780768313 @default.
- W1480930112 hasConceptScore W1480930112C28859421 @default.
- W1480930112 hasConceptScore W1480930112C54355233 @default.
- W1480930112 hasConceptScore W1480930112C55493867 @default.
- W1480930112 hasConceptScore W1480930112C86803240 @default.
- W1480930112 hasConceptScore W1480930112C95444343 @default.
- W1480930112 hasIssue "12" @default.
- W1480930112 hasLocation W14809301121 @default.
- W1480930112 hasOpenAccess W1480930112 @default.
- W1480930112 hasPrimaryLocation W14809301121 @default.
- W1480930112 hasRelatedWork W1566729005 @default.
- W1480930112 hasRelatedWork W1700716001 @default.
- W1480930112 hasRelatedWork W1981381991 @default.
- W1480930112 hasRelatedWork W2013908504 @default.
- W1480930112 hasRelatedWork W2054843986 @default.
- W1480930112 hasRelatedWork W2116210813 @default.
- W1480930112 hasRelatedWork W2138350991 @default.
- W1480930112 hasRelatedWork W2409285845 @default.
- W1480930112 hasRelatedWork W2434195791 @default.
- W1480930112 hasRelatedWork W3015376786 @default.
- W1480930112 hasVolume "250" @default.
- W1480930112 isParatext "false" @default.
- W1480930112 isRetracted "false" @default.
- W1480930112 magId "1480930112" @default.
- W1480930112 workType "article" @default.