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• W1481162520 abstract "Mild acid degradation of the lipopolysaccharide of Citro- bacter gillenii O9a,9b released a polysaccharide (PS), which was found to consist of a single monosaccharide, 4-acetamido-4,6-dideoxy-d-mannose (d-Rha4NAc, N-acetyl-d-perosamine). PS was studied by methylation analysis␣and 1H-NMR and 13C-NMR spectroscopy, using two-dimensional 1H,1H COSY, TOCSY, NOESY, and H-detected 1H,13C heteronuclear correlation experiments. It was␣found that PS includes two structurally different polysaccharides: an α1→2-linked homopolymer of N-acetyl-d-perosamine [→2)-α-d-Rhap4NAc-(1→, PS2] and a␣polysaccharide composed of tetrasaccharide repeating units (PS1) with the␣following structure: →3)-α-d-Rhap4NAc-(1→2)-α-d-Rhap4NAc-(1→2)-α-d-Rhap4NAc-(1→3)-α-d-Rhap4N Ac2Ac-(1→ where the degree of O-acetylation of a 3-substituted Rha4NAc residue at position 2 is ≈ 70%. PS could be fractionated into PS1 and PS2 by gel-permeation chromatography on TSK HW-50S. Matrix-assisted laser desorption ionization MS data indicate sequential chain elongation of both PS1 and PS2 by a single sugar unit, with O-acetylation in PS1 beginning at a certain chain length. Anti-(C. gillenii O9a,9b) serum reacted with PS1 in double immunodiffusion and immunoblotting, whereas neither PS2 nor the lipopolysaccharide of Vibrio cholerae O1 with a structurally related O-chain polysaccharide were reactive." @default.
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• W1481162520 date "2002-01-01" @default.
• W1481162520 modified "2023-09-25" @default.
• W1481162520 title "Structures of two O-chain polysaccharides of Citrobacter gillenii O9a,9b lipopolysaccharide" @default.
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• W1481162520 doi "https://doi.org/10.1046/j.0014-2956.2001.02638.x" @default.
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